Zinc oxide nanoparticles improve gut health and reduce faecal zinc excretion in piglets

Yiyan Cui, Zhimei Tian, Huijie Lu, Dun Deng, Zhichang Liu, Ting Rong, Miao Yu, Xianyong Ma

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5 Scopus citations


This study investigated the effects of zinc oxide nanoparticles (ZnO–N) on growth performance, gut health, and zinc and copper excretion in piglets. A total of 144 piglets (Duroc × Landrace × Large White, weaned at 21 days; mean initial weight 6.15 ± 0.01 kg) were assigned to six groups with six replicates of four pigs per pen for a 26-day feeding trial. The groups were: antibiotic group (basal diet + 75 mg/kg aureomycin), ZnO group (basal diet + 1,600 mg/kg zinc oxide), and four ZnO–N groups (basal diet + 200, 300, 400, or 500 mg/kg ZnO–N, respectively). No significant difference in growth performance was observed amongst the treatments. ZnO–N supplementation improved the activities of superoxide dismutase (linear or quadratic, p < 0.05), copper-zinc superoxide dismutase in the ileum (linear, p < 0.05), catalase in the jejunum and ileum (linear or quadratic, p < 0.05), total antioxidant capacity of duodenum and ileum (linear, p < 0.05), and glutathione peroxidase in the ileum (linear, p < 0.05), and reduced malondialdehyde content in the jejunum (linear, p < 0.05). As ZnO–N content was increased, the contents of jejunal IgA, interleukin (IL)-6, and IL-10 decreased (linear, p < 0.05), while IgM content increased (linear, p < 0.05). The contents of ileal IgA and IL-10 increased (linear and quadratic, p < 0.05), while the contents of IgM, IgG, and IL-6 decreased (linear or quadratic, p < 0.05). Villus height and the ratio of villus height to crypt depth in piglets fed with ZnO–N were higher than in the antibiotic group (p < 0.05). Pigs fed with 200–500 mg/kg ZnO–N showed lower contents of hepatic zinc and copper and faecal zinc than the ZnO group (p < 0.05). These results show that dietary ZnO–N enhanced intestinal antioxidant and immune capacity and intestinal morphology, particularly 500 mg/kg ZnO–N, and also that ZnO–N minimised zinc content in the liver and faeces.

Original languageEnglish
Article number104610
JournalLivestock Science
StatePublished - Sep 2021
Externally publishedYes


  • Gut health
  • Piglets
  • Zinc
  • Zinc oxide nanoparticles


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