@article{18f1cf7a95864b23b2756c6a2615ea1b,
title = "Zika virus tissue and blood compartmentalization in acute infection of rhesus macaques",
abstract = "Animal models of Zika virus (ZIKV) are needed to better understand tropism and pathogenesis and to test candidate vaccines and therapies to curtail the pandemic. Humans and rhesus macaques possess similar fetal development and placental biology that is not shared between humans and rodents. We inoculated 2 non-pregnant rhesus macaques with a 2015 Brazilian ZIKV strain. Consistent with most human infections, the animals experienced no clinical disease but developed short-lived plasma viremias that cleared as neutralizing antibody developed. In 1 animal, viral RNA (vRNA) could be detected longer in whole blood than in plasma. Despite no major histopathologic changes, many adult tissues contained vRNA 14 days post-infection with highest levels in hemolymphatic tissues. These observations warrant further studies to investigate ZIKV persistence and its potential clinical implications for transmission via blood products or tissue and organ transplants.",
author = "Coffey, {Lark L.} and Pesavento, {Patricia A.} and Keesler, {Rebekah I.} and Anil Singapuri and Jennifer Watanabe and Rie Watanabe and Yee, {Jo Ann} and Eliza Bliss-Moreau and Christina Cruzen and Christe, {Kari L.} and Reader, {J. Rachel} and {Von Morgenland}, Wilhelm and Gibbons, {Anne M.} and Allen, {A. Mark} and Jeff Linnen and Kui Gao and Eric Delwart and Graham Simmons and Mars Stone and Marion Lanteri and Sonia Bakkour and Michael Busch and John Morrison and {Van Rompay}, {Koen K.A.}",
note = "Funding Information: We acknowledge the research and support staff at CNPRC for their assistance with all aspects of the study, as well as the UC Davis IACUC and Environmental Health and Safety Offices for expedited approval for work on this project. Sarah Mills, Abigail Spinner and the staff of the Pathogen Detection Laboratory and Clinical Laboratories at CNPRC were especially helpful with necropsies and biospecimen processing, and analysis. Kathy West provided illustrations. The Zika research team at the Wisconsin National Primate Research Center provided ongoing advice and data-sharing. The Unit{\'e} des Virus Emergents, Marseille, France, provided the inactivated French Polynesia 2013 standard. This study was supported by a CNPRC pilot research grant to LC and KVR, the Office of Research Infrastructure Programs/OD (P51OD011107), and start up funds from the Pathology, Microbiology and Immunology Department to LC. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Although JL and KG are employees of Hologic, their role in the study was to test samples from macaques. Hologic did not provide any funding or salary support for other manuscript co-authors. Publisher Copyright: {\textcopyright} 2017 Coffey et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",
year = "2017",
month = jan,
doi = "10.1371/journal.pone.0171148",
language = "English",
volume = "12",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "1",
}