Xylosyltransferase 2 deficiency and organ homeostasis

Beatrix Ferencz, Eduard Condac, Nabin Poudel, Maria Cristina Munteanu, Pulavendran Sivasami, Biswa Choudhury, Nandita Natasha Naidu, Fuming Zhang, Melanie Breshears, Robert J. Linhardt, Myron E. Hinsdale

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

In this paper we characterize the function of Xylosyltransferase 2 (XylT2) in different tissues to investigate the role XylT2 has in the proteoglycan (PG) biochemistry of multiple organs. The results show that in all organs examined there is a widespread and significant decrease in total XylT activity in Xylt2 knock out mice (Xylt2−/−). This decrease results in increased organ weight differences in lung, heart, and spleen. These findings, in addition to our previous findings of increased liver and kidney weight with loss of serum XylT activity, suggest systemic changes in organ function due to loss of XylT2 activity. The Xylt2−/− mice have splenomegaly due to enlargement of the red pulp area and enhanced pulmonary response to bacterial liposaccharide. Tissue glycosaminoglycan composition changes are also found. These results demonstrate a role of XylT2 activity in multiple organs and their PG content. Because the residual XylT activity in the Xylt2−/− is due to xylosyltransferase 1 (XylT1), these studies indicate that both XylT1 and XylT2 have important roles in PG biosynthesis and organ homeostasis.

Original languageEnglish
Pages (from-to)755-765
Number of pages11
JournalGlycoconjugate Journal
Volume37
Issue number6
DOIs
StatePublished - Dec 2020
Externally publishedYes

Keywords

  • Disaccharide analyses
  • Extracellular matrix
  • Genetic modifier
  • Glycosaminoglycans
  • Glycotransferase
  • Organ homeostasis
  • Proteoglycans
  • Proteoglycans
  • Xylosyltransferase

Fingerprint

Dive into the research topics of 'Xylosyltransferase 2 deficiency and organ homeostasis'. Together they form a unique fingerprint.

Cite this