Xwnt-2b is a novel axis-inducing Xenopus Wnt, which is expressed in embryonic brain

Yosef Landesman; Sergei Y. Sokol

doi:10.1016/S0925-4773(97)00041-5

Xwnt-2b is a novel axis-inducing Xenopus Wnt, which is expressed in embryonic brain

Yosef Landesman
, Sergei Y. Sokol

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

Xwnt-2b is a novel member of the Wnt gene family and is 73-74% similar to human and mouse Wnt-2 proteins. Starting from stage 15, Xwnt-2b transcripts are localized to a non-contiguous stripe in the anterior neural plate of the Xenopus embryo. In the tailbud, Xwnt-2b is expressed along the dorsoanterior side of the prosencephalon-mesencephalon boundary. At the tadpole stages, the brain-specific expression fades, but the total amount of Xwnt-2b mRNA does not decline due to activation of its expression in non- brain areas. Microinjection of Xwnt-2b mRNA into a ventral blastomere of 4- 8-cell embryos results in the formation of complete secondary body axes. These results suggest that Xwnt-2b is a member of the axis-inducing Writs and that it is involved in brain development and in later organogenesis.

Original language	English
Pages (from-to)	199-209
Number of pages	11
Journal	Mechanisms of Development
Volume	63
Issue number	2
DOIs	https://doi.org/10.1016/S0925-4773(97)00041-5
State	Published - May 1997
Externally published	Yes

Keywords

Brain development
Dorsoventral axis
En-2
Neural induction
Wnt
Xenopus

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title = "Xwnt-2b is a novel axis-inducing Xenopus Wnt, which is expressed in embryonic brain",

abstract = "Xwnt-2b is a novel member of the Wnt gene family and is 73-74\% similar to human and mouse Wnt-2 proteins. Starting from stage 15, Xwnt-2b transcripts are localized to a non-contiguous stripe in the anterior neural plate of the Xenopus embryo. In the tailbud, Xwnt-2b is expressed along the dorsoanterior side of the prosencephalon-mesencephalon boundary. At the tadpole stages, the brain-specific expression fades, but the total amount of Xwnt-2b mRNA does not decline due to activation of its expression in non- brain areas. Microinjection of Xwnt-2b mRNA into a ventral blastomere of 4- 8-cell embryos results in the formation of complete secondary body axes. These results suggest that Xwnt-2b is a member of the axis-inducing Writs and that it is involved in brain development and in later organogenesis.",

keywords = "Brain development, Dorsoventral axis, En-2, Neural induction, Wnt, Xenopus",

author = "Yosef Landesman and Sokol, \{Sergei Y.\}",

note = "Funding Information: We thank A. Hemmati-Brivanlou for En-2 plasmid and A. McMahon for human Wnt-2 plasmid. We are grateful to J. Green, M. Mercola, T. Holowacz, K. Itoh, A. O'Reilly, B. Turano, M. Fan, C. Vandeburg and M. Kapushchevsky for helpful discussions and for critical reviewing of this manuscript. We acknowledge support from D. Melton at the early stages of this project. The work reported in this paper was undertaken during the tenure of a Research Training Fellowship awarded by the International Agency for Research on Cancer to Y.L. This study was supported by the March of Dimes Birth Defects Foundation and by the NIH grants to S.Y.S.",

year = "1997",

month = may,

doi = "10.1016/S0925-4773(97)00041-5",

language = "English",

volume = "63",

pages = "199--209",

journal = "Mechanisms of Development",

issn = "0925-4773",

publisher = "Elsevier B.V.",

number = "2",

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AU - Landesman, Yosef

AU - Sokol, Sergei Y.

N1 - Funding Information: We thank A. Hemmati-Brivanlou for En-2 plasmid and A. McMahon for human Wnt-2 plasmid. We are grateful to J. Green, M. Mercola, T. Holowacz, K. Itoh, A. O'Reilly, B. Turano, M. Fan, C. Vandeburg and M. Kapushchevsky for helpful discussions and for critical reviewing of this manuscript. We acknowledge support from D. Melton at the early stages of this project. The work reported in this paper was undertaken during the tenure of a Research Training Fellowship awarded by the International Agency for Research on Cancer to Y.L. This study was supported by the March of Dimes Birth Defects Foundation and by the NIH grants to S.Y.S.

PY - 1997/5

Y1 - 1997/5

N2 - Xwnt-2b is a novel member of the Wnt gene family and is 73-74% similar to human and mouse Wnt-2 proteins. Starting from stage 15, Xwnt-2b transcripts are localized to a non-contiguous stripe in the anterior neural plate of the Xenopus embryo. In the tailbud, Xwnt-2b is expressed along the dorsoanterior side of the prosencephalon-mesencephalon boundary. At the tadpole stages, the brain-specific expression fades, but the total amount of Xwnt-2b mRNA does not decline due to activation of its expression in non- brain areas. Microinjection of Xwnt-2b mRNA into a ventral blastomere of 4- 8-cell embryos results in the formation of complete secondary body axes. These results suggest that Xwnt-2b is a member of the axis-inducing Writs and that it is involved in brain development and in later organogenesis.

AB - Xwnt-2b is a novel member of the Wnt gene family and is 73-74% similar to human and mouse Wnt-2 proteins. Starting from stage 15, Xwnt-2b transcripts are localized to a non-contiguous stripe in the anterior neural plate of the Xenopus embryo. In the tailbud, Xwnt-2b is expressed along the dorsoanterior side of the prosencephalon-mesencephalon boundary. At the tadpole stages, the brain-specific expression fades, but the total amount of Xwnt-2b mRNA does not decline due to activation of its expression in non- brain areas. Microinjection of Xwnt-2b mRNA into a ventral blastomere of 4- 8-cell embryos results in the formation of complete secondary body axes. These results suggest that Xwnt-2b is a member of the axis-inducing Writs and that it is involved in brain development and in later organogenesis.

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KW - En-2

KW - Neural induction

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KW - Xenopus

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ER -

Xwnt-2b is a novel axis-inducing Xenopus Wnt, which is expressed in embryonic brain

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Keywords

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