TY - CHAP
T1 - X-Ray Diffraction Experiment
T2 - The Last Experiment in the Structure Elucidation Process
AU - Domagalski, Marcin
AU - Borek, Dominika
AU - Otwinowski, Zbyszek
AU - Chruszcz, Maksymilian
AU - Minor, Wladek
PY - 2009
Y1 - 2009
N2 - Recent years have brought not only an avalanche of new macromolecular structures, but also significant advances in the protein structure determination methodology only now making its way into structure-based drug discovery. In this chapter, we review recent methodology developments in X-ray diffraction experiments that led to fast and very accurate elucidation of three-dimensional structures of macromolecules. We will discuss the role of data collection as the last experiment performed in the crystal structure determination process. A statistical analysis of diffraction experiments that are reported in the Protein Data Bank (PDB) is also presented.
AB - Recent years have brought not only an avalanche of new macromolecular structures, but also significant advances in the protein structure determination methodology only now making its way into structure-based drug discovery. In this chapter, we review recent methodology developments in X-ray diffraction experiments that led to fast and very accurate elucidation of three-dimensional structures of macromolecules. We will discuss the role of data collection as the last experiment performed in the crystal structure determination process. A statistical analysis of diffraction experiments that are reported in the Protein Data Bank (PDB) is also presented.
UR - http://www.scopus.com/inward/record.url?scp=78049421630&partnerID=8YFLogxK
U2 - 10.1016/S1876-1623(09)77002-6
DO - 10.1016/S1876-1623(09)77002-6
M3 - Chapter
C2 - 20663480
AN - SCOPUS:78049421630
T3 - Advances in Protein Chemistry and Structural Biology
SP - 23
EP - 40
BT - Advances in Protein Chemistry and Structural Biology
ER -