TY - JOUR
T1 - Wolff–Parkinson–White syndrome
T2 - De novo variants and evidence for mutational burden in genes associated with atrial fibrillation
AU - Coban-Akdemir, Zeynep H.
AU - Charng, Wu Lin
AU - Azamian, Mahshid
AU - Paine, Ingrid S.
AU - Punetha, Jaya
AU - Grochowski, Christopher M.
AU - Gambin, Tomasz
AU - Valdes, Santiago O.
AU - Cannon, Bryan
AU - Zapata, Gladys
AU - Hernandez, Patricia P.
AU - Jhangiani, Shalini
AU - Doddapaneni, Harsha
AU - Hu, Jianhong
AU - Boricha, Fatima
AU - Muzny, Donna M.
AU - Boerwinkle, Eric
AU - Yang, Yaping
AU - Gibbs, Richard A.
AU - Posey, Jennifer E.
AU - Wehrens, Xander H.T.
AU - Belmont, John W.
AU - Kim, Jeffrey J.
AU - Miyake, Christina Y.
AU - Lupski, James R.
AU - Lalani, Seema R.
N1 - Publisher Copyright:
© 2020 Wiley Periodicals, Inc.
PY - 2020/6/1
Y1 - 2020/6/1
N2 - Background: Wolff–Parkinson–White (WPW) syndrome is a relatively common arrhythmia affecting ~1–3/1,000 individuals. Mutations in PRKAG2 have been described in rare patients in association with cardiomyopathy. However, the genetic basis of WPW in individuals with a structurally normal heart remains poorly understood. Sudden death due to atrial fibrillation (AF) can also occur in these individuals. Several studies have indicated that despite ablation of an accessory pathway, the risk of AF remains high in patients compared to general population. Methods: We applied exome sequencing in 305 subjects, including 65 trios, 80 singletons, and 6 multiple affected families. We used de novo analysis, candidate gene approach, and burden testing to explore the genetic contributions to WPW. Results: A heterozygous deleterious variant in PRKAG2 was identified in one subject, accounting for 0.6% (1/151) of the genetic basis of WPW in this study. Another individual with WPW and left ventricular hypertrophy carried a known pathogenic variant in MYH7. We found rare de novo variants in genes associated with arrhythmia and cardiomyopathy (ANK2, NEBL, PITX2, and PRDM16) in this cohort. There was an increased burden of rare deleterious variants (MAF ≤ 0.005) with CADD score ≥ 25 in genes linked to AF in cases compared to controls (P =.0023). Conclusions: Our findings show an increased burden of rare deleterious variants in genes linked to AF in WPW syndrome, suggesting that genetic factors that determine the development of accessory pathways may be linked to an increased susceptibility of atrial muscle to AF in a subset of patients.
AB - Background: Wolff–Parkinson–White (WPW) syndrome is a relatively common arrhythmia affecting ~1–3/1,000 individuals. Mutations in PRKAG2 have been described in rare patients in association with cardiomyopathy. However, the genetic basis of WPW in individuals with a structurally normal heart remains poorly understood. Sudden death due to atrial fibrillation (AF) can also occur in these individuals. Several studies have indicated that despite ablation of an accessory pathway, the risk of AF remains high in patients compared to general population. Methods: We applied exome sequencing in 305 subjects, including 65 trios, 80 singletons, and 6 multiple affected families. We used de novo analysis, candidate gene approach, and burden testing to explore the genetic contributions to WPW. Results: A heterozygous deleterious variant in PRKAG2 was identified in one subject, accounting for 0.6% (1/151) of the genetic basis of WPW in this study. Another individual with WPW and left ventricular hypertrophy carried a known pathogenic variant in MYH7. We found rare de novo variants in genes associated with arrhythmia and cardiomyopathy (ANK2, NEBL, PITX2, and PRDM16) in this cohort. There was an increased burden of rare deleterious variants (MAF ≤ 0.005) with CADD score ≥ 25 in genes linked to AF in cases compared to controls (P =.0023). Conclusions: Our findings show an increased burden of rare deleterious variants in genes linked to AF in WPW syndrome, suggesting that genetic factors that determine the development of accessory pathways may be linked to an increased susceptibility of atrial muscle to AF in a subset of patients.
KW - ANK2
KW - Wolff–Parkinson–White (WPW) syndrome
KW - atrial fibrillation
KW - exome sequencing
UR - http://www.scopus.com/inward/record.url?scp=85082600547&partnerID=8YFLogxK
U2 - 10.1002/ajmg.a.61571
DO - 10.1002/ajmg.a.61571
M3 - Article
C2 - 32233023
AN - SCOPUS:85082600547
SN - 1552-4825
VL - 182
SP - 1387
EP - 1399
JO - American Journal of Medical Genetics, Part A
JF - American Journal of Medical Genetics, Part A
IS - 6
ER -