Whole-genome sequencing identifies emergence of a quinolone resistance mutation in a case of stenotrophomonas maltophilia bacteremia

Theodore R. Pak; Deena R. Altman; Oliver Attie; Robert Sebra; Camille L. Hamula; Martha Lewis; Gintaras Deikus; Leah C. Newman; Gang Fang; Jonathan Hand; Gopi Patel; Fran Wallach; Eric E. Schadt; Shirish Huprikar; Harm Van Bakel; Andrew Kasarskis; Ali Bashir

doi:10.1128/AAC.01723-15

Whole-genome sequencing identifies emergence of a quinolone resistance mutation in a case of stenotrophomonas maltophilia bacteremia

Theodore R. Pak
, Deena R. Altman
, Oliver Attie
, Robert Sebra
, Camille L. Hamula
, Martha Lewis
, Gintaras Deikus
, Leah C. Newman
, Gang Fang
, Jonathan Hand
, Gopi Patel
, Fran Wallach
, Eric E. Schadt
, Shirish Huprikar
, Harm Van Bakel
, Andrew Kasarskis
, Ali Bashir

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Whole-genome sequences for Stenotrophomonas maltophilia serial isolates from a bacteremic patient before and after development of levofloxacin resistance were assembled de novo and differed by one single-nucleotide variant in smeT, a repressor for multidrug efflux operon smeDEF. Along with sequenced isolates from five contemporaneous cases, they displayed considerable diversity compared against all published complete genomes. Whole-genome sequencing and complete assembly can conclusively identify resistance mechanisms emerging in S. maltophilia strains during clinical therapy.

Original language	English
Pages (from-to)	7117-7120
Number of pages	4
Journal	Antimicrobial Agents and Chemotherapy
Volume	59
Issue number	11
DOIs	https://doi.org/10.1128/AAC.01723-15
State	Published - 1 Nov 2015

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Pak, T. R., Altman, D. R., Attie, O., Sebra, R., Hamula, C. L., Lewis, M., Deikus, G., Newman, L. C., Fang, G., Hand, J., Patel, G., Wallach, F., Schadt, E. E., Huprikar, S., Van Bakel, H., Kasarskis, A., & Bashir, A. (2015). Whole-genome sequencing identifies emergence of a quinolone resistance mutation in a case of stenotrophomonas maltophilia bacteremia. Antimicrobial Agents and Chemotherapy, 59(11), 7117-7120. https://doi.org/10.1128/AAC.01723-15

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title = "Whole-genome sequencing identifies emergence of a quinolone resistance mutation in a case of stenotrophomonas maltophilia bacteremia",

abstract = "Whole-genome sequences for Stenotrophomonas maltophilia serial isolates from a bacteremic patient before and after development of levofloxacin resistance were assembled de novo and differed by one single-nucleotide variant in smeT, a repressor for multidrug efflux operon smeDEF. Along with sequenced isolates from five contemporaneous cases, they displayed considerable diversity compared against all published complete genomes. Whole-genome sequencing and complete assembly can conclusively identify resistance mechanisms emerging in S. maltophilia strains during clinical therapy.",

author = "Pak, \{Theodore R.\} and Altman, \{Deena R.\} and Oliver Attie and Robert Sebra and Hamula, \{Camille L.\} and Martha Lewis and Gintaras Deikus and Newman, \{Leah C.\} and Gang Fang and Jonathan Hand and Gopi Patel and Fran Wallach and Schadt, \{Eric E.\} and Shirish Huprikar and \{Van Bakel\}, Harm and Andrew Kasarskis and Ali Bashir",

year = "2015",

month = nov,

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doi = "10.1128/AAC.01723-15",

language = "English",

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pages = "7117--7120",

journal = "Antimicrobial Agents and Chemotherapy",

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Pak, TR, Altman, DR, Attie, O, Sebra, R, Hamula, CL, Lewis, M, Deikus, G, Newman, LC, Fang, G, Hand, J, Patel, G, Wallach, F, Schadt, EE , Huprikar, S , Van Bakel, H , Kasarskis, A & Bashir, A 2015, 'Whole-genome sequencing identifies emergence of a quinolone resistance mutation in a case of stenotrophomonas maltophilia bacteremia', Antimicrobial Agents and Chemotherapy, vol. 59, no. 11, pp. 7117-7120. https://doi.org/10.1128/AAC.01723-15

TY - JOUR

T1 - Whole-genome sequencing identifies emergence of a quinolone resistance mutation in a case of stenotrophomonas maltophilia bacteremia

AU - Pak, Theodore R.

AU - Altman, Deena R.

AU - Attie, Oliver

AU - Sebra, Robert

AU - Hamula, Camille L.

AU - Lewis, Martha

AU - Deikus, Gintaras

AU - Newman, Leah C.

AU - Fang, Gang

AU - Hand, Jonathan

AU - Patel, Gopi

AU - Wallach, Fran

AU - Schadt, Eric E.

AU - Huprikar, Shirish

AU - Van Bakel, Harm

AU - Kasarskis, Andrew

AU - Bashir, Ali

PY - 2015/11/1

Y1 - 2015/11/1

N2 - Whole-genome sequences for Stenotrophomonas maltophilia serial isolates from a bacteremic patient before and after development of levofloxacin resistance were assembled de novo and differed by one single-nucleotide variant in smeT, a repressor for multidrug efflux operon smeDEF. Along with sequenced isolates from five contemporaneous cases, they displayed considerable diversity compared against all published complete genomes. Whole-genome sequencing and complete assembly can conclusively identify resistance mechanisms emerging in S. maltophilia strains during clinical therapy.

AB - Whole-genome sequences for Stenotrophomonas maltophilia serial isolates from a bacteremic patient before and after development of levofloxacin resistance were assembled de novo and differed by one single-nucleotide variant in smeT, a repressor for multidrug efflux operon smeDEF. Along with sequenced isolates from five contemporaneous cases, they displayed considerable diversity compared against all published complete genomes. Whole-genome sequencing and complete assembly can conclusively identify resistance mechanisms emerging in S. maltophilia strains during clinical therapy.

UR - https://www.scopus.com/pages/publications/84946219358

U2 - 10.1128/AAC.01723-15

DO - 10.1128/AAC.01723-15

M3 - Article

C2 - 26324280

AN - SCOPUS:84946219358

SN - 0066-4804

VL - 59

SP - 7117

EP - 7120

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 11

ER -

Whole-genome sequencing identifies emergence of a quinolone resistance mutation in a case of stenotrophomonas maltophilia bacteremia

Abstract

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