Whole-genome sequencing identifies emergence of a quinolone resistance mutation in a case of stenotrophomonas maltophilia bacteremia

Theodore R. Pak, Deena R. Altman, Oliver Attie, Robert Sebra, Camille L. Hamula, Martha Lewis, Gintaras Deikus, Leah C. Newman, Gang Fang, Jonathan Hand, Gopi Patel, Fran Wallach, Eric E. Schadt, Shirish Huprikar, Harm Van Bakel, Andrew Kasarskis, Ali Bashir

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Whole-genome sequences for Stenotrophomonas maltophilia serial isolates from a bacteremic patient before and after development of levofloxacin resistance were assembled de novo and differed by one single-nucleotide variant in smeT, a repressor for multidrug efflux operon smeDEF. Along with sequenced isolates from five contemporaneous cases, they displayed considerable diversity compared against all published complete genomes. Whole-genome sequencing and complete assembly can conclusively identify resistance mechanisms emerging in S. maltophilia strains during clinical therapy.

Original languageEnglish
Pages (from-to)7117-7120
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume59
Issue number11
DOIs
StatePublished - 1 Nov 2015

Fingerprint

Dive into the research topics of 'Whole-genome sequencing identifies emergence of a quinolone resistance mutation in a case of stenotrophomonas maltophilia bacteremia'. Together they form a unique fingerprint.

Cite this