Whole-genome sequencing identifies emergence of a quinolone resistance mutation in a case of stenotrophomonas maltophilia bacteremia

Theodore R. Pak; Deena R. Altman; Oliver Attie; Robert Sebra; Camille L. Hamula; Martha Lewis; Gintaras Deikus; Leah C. Newman; Gang Fang; Jonathan Hand; Gopi Patel; Fran Wallach; Eric E. Schadt; Shirish Huprikar; Harm Van Bakel; Andrew Kasarskis; Ali Bashir

doi:10.1128/AAC.01723-15

Whole-genome sequencing identifies emergence of a quinolone resistance mutation in a case of stenotrophomonas maltophilia bacteremia

Theodore R. Pak, Deena R. Altman, Oliver Attie, Robert Sebra, Camille L. Hamula, Martha Lewis, Gintaras Deikus, Leah C. Newman, Gang Fang, Jonathan Hand, Gopi Patel, Fran Wallach, Eric E. Schadt, Shirish Huprikar, Harm Van Bakel, Andrew Kasarskis, Ali Bashir

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17 Scopus citations

Abstract

Whole-genome sequences for Stenotrophomonas maltophilia serial isolates from a bacteremic patient before and after development of levofloxacin resistance were assembled de novo and differed by one single-nucleotide variant in smeT, a repressor for multidrug efflux operon smeDEF. Along with sequenced isolates from five contemporaneous cases, they displayed considerable diversity compared against all published complete genomes. Whole-genome sequencing and complete assembly can conclusively identify resistance mechanisms emerging in S. maltophilia strains during clinical therapy.

Original language	English
Pages (from-to)	7117-7120
Number of pages	4
Journal	Antimicrobial Agents and Chemotherapy
Volume	59
Issue number	11
DOIs	https://doi.org/10.1128/AAC.01723-15
State	Published - 1 Nov 2015

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Pak, T. R., Altman, D. R., Attie, O., Sebra, R., Hamula, C. L., Lewis, M., Deikus, G., Newman, L. C., Fang, G., Hand, J., Patel, G., Wallach, F., Schadt, E. E., Huprikar, S., Van Bakel, H., Kasarskis, A., & Bashir, A. (2015). Whole-genome sequencing identifies emergence of a quinolone resistance mutation in a case of stenotrophomonas maltophilia bacteremia. Antimicrobial Agents and Chemotherapy, 59(11), 7117-7120. https://doi.org/10.1128/AAC.01723-15

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title = "Whole-genome sequencing identifies emergence of a quinolone resistance mutation in a case of stenotrophomonas maltophilia bacteremia",

abstract = "Whole-genome sequences for Stenotrophomonas maltophilia serial isolates from a bacteremic patient before and after development of levofloxacin resistance were assembled de novo and differed by one single-nucleotide variant in smeT, a repressor for multidrug efflux operon smeDEF. Along with sequenced isolates from five contemporaneous cases, they displayed considerable diversity compared against all published complete genomes. Whole-genome sequencing and complete assembly can conclusively identify resistance mechanisms emerging in S. maltophilia strains during clinical therapy.",

author = "Pak, {Theodore R.} and Altman, {Deena R.} and Oliver Attie and Robert Sebra and Hamula, {Camille L.} and Martha Lewis and Gintaras Deikus and Newman, {Leah C.} and Gang Fang and Jonathan Hand and Gopi Patel and Fran Wallach and Schadt, {Eric E.} and Shirish Huprikar and {Van Bakel}, Harm and Andrew Kasarskis and Ali Bashir",

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doi = "10.1128/AAC.01723-15",

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Pak, TR, Altman, DR, Attie, O, Sebra, R, Hamula, CL, Lewis, M, Deikus, G, Newman, LC, Fang, G, Hand, J, Patel, G, Wallach, F, Schadt, EE , Huprikar, S , Van Bakel, H , Kasarskis, A & Bashir, A 2015, 'Whole-genome sequencing identifies emergence of a quinolone resistance mutation in a case of stenotrophomonas maltophilia bacteremia', Antimicrobial Agents and Chemotherapy, vol. 59, no. 11, pp. 7117-7120. https://doi.org/10.1128/AAC.01723-15

TY - JOUR

T1 - Whole-genome sequencing identifies emergence of a quinolone resistance mutation in a case of stenotrophomonas maltophilia bacteremia

AU - Pak, Theodore R.

AU - Altman, Deena R.

AU - Attie, Oliver

AU - Sebra, Robert

AU - Hamula, Camille L.

AU - Lewis, Martha

AU - Deikus, Gintaras

AU - Newman, Leah C.

AU - Fang, Gang

AU - Hand, Jonathan

AU - Patel, Gopi

AU - Wallach, Fran

AU - Schadt, Eric E.

AU - Huprikar, Shirish

AU - Van Bakel, Harm

AU - Kasarskis, Andrew

AU - Bashir, Ali

PY - 2015/11/1

Y1 - 2015/11/1

N2 - Whole-genome sequences for Stenotrophomonas maltophilia serial isolates from a bacteremic patient before and after development of levofloxacin resistance were assembled de novo and differed by one single-nucleotide variant in smeT, a repressor for multidrug efflux operon smeDEF. Along with sequenced isolates from five contemporaneous cases, they displayed considerable diversity compared against all published complete genomes. Whole-genome sequencing and complete assembly can conclusively identify resistance mechanisms emerging in S. maltophilia strains during clinical therapy.

AB - Whole-genome sequences for Stenotrophomonas maltophilia serial isolates from a bacteremic patient before and after development of levofloxacin resistance were assembled de novo and differed by one single-nucleotide variant in smeT, a repressor for multidrug efflux operon smeDEF. Along with sequenced isolates from five contemporaneous cases, they displayed considerable diversity compared against all published complete genomes. Whole-genome sequencing and complete assembly can conclusively identify resistance mechanisms emerging in S. maltophilia strains during clinical therapy.

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U2 - 10.1128/AAC.01723-15

DO - 10.1128/AAC.01723-15

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AN - SCOPUS:84946219358

SN - 0066-4804

VL - 59

SP - 7117

EP - 7120

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 11

ER -

Whole-genome sequencing identifies emergence of a quinolone resistance mutation in a case of stenotrophomonas maltophilia bacteremia

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