Water-soluble carbon nanotube-enzyme conjugates as functional biocatalytic formulations

Prashanth Asuri, Sandeep S. Karajanagi, Edward Sellitto, Dae Yun Kim, Ravi S. Kane, Jonathan S. Dordick

Research output: Contribution to journalArticlepeer-review

150 Scopus citations

Abstract

We report the activity, stability, and reusability of enzyme-carbon nanotube conjugates in aqueous solutions. A variety of enzymes were covalently attached to oxidized multi-walled carbon nanotubes (MWNTs). These conjugates were soluble in aqueous buffer, retained a high fraction of their native activity, and were stable at higher temperatures relative to their solution phase counterparts. Furthermore, the high surface area of MWNTs afforded high enzyme loadings, yet the intrinsic high length of the MWNT led to facile filtration. These water-soluble carbon nanotube-enzyme conjugates represent novel preparations that possess the virtues of both soluble and immobilized enzymes, thus providing a unique combination of useful attributes such as low mass transfer resistance, high activity and stability, and reusability.

Original languageEnglish
Pages (from-to)804-811
Number of pages8
JournalBiotechnology and Bioengineering
Volume95
Issue number5
DOIs
StatePublished - 5 Dec 2006
Externally publishedYes

Keywords

  • Caspase inhibition
  • Enzyme stabilization
  • Enzyme-nanotube conjugates
  • Reusability

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