Abstract
Simple and informative method for the elucidation of de novo synthesized forms of microsomal cytochrome P-448 induced by 3-methylcholanthrene and 2,3,7,8-tetrachlordibenzo-p-dioxine has been developed. The method is based on gel fluorography upon electrophoretic separation of microsomal proteins obtained from the liver of rats pre-treated with the inducers of monooxygenase system components and then with 14C-leucine. At least two forms of cytochrome P-448 (with molecular weight of 56000 and 53000) were shown to be de novo synthesized under the influence of 3-methylcholanthrene and 2,3,7,8-tetrachlodbibenzo-p-dioxine.
| Translated title of the contribution | Detection of synthesized microsomal hemoproteins (cytochrome P-448) using autofluorography |
|---|---|
| Original language | Russian |
| Pages (from-to) | 29-30 |
| Number of pages | 2 |
| Journal | Byulleten Eksperimentalnoi Biologii i Meditsiny |
| Volume | 101 |
| Issue number | 1 |
| State | Published - Jan 1986 |