Visualizing the balbiani body in Zebrafish oocytes

Kathy Ann L. Lee, Florence L. Marlow

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

3 Scopus citations

Abstract

Approaches to visualize the Balbiani body of zebrafish primary oocytes using protein, RNA, and mitochondrial markers are described. The method involves isolation, histology, staining, and microscopic examination of early zebrafish oocytes. These techniques can be applied to visualize gene products that are localized to the Balbiani body, and when applied to mutants can be used to decipher molecular and genetic pathways acting in Balbiani body development in early oocytes.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages277-293
Number of pages17
DOIs
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
Volume1920
ISSN (Print)1064-3745

Keywords

  • Balbiani body
  • Buckyball
  • Histology
  • Immunohistochemistry
  • Microscopy
  • Mitochondria
  • Oocyte
  • Primary oocytes
  • RNA and protein localization

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