Visualization ex situ of single DNA molecules incubation: A first step for quantitative analysis on multi-site degradation and enzymatic kinetics

Xinyan Wang; Haijun Yang; Huabin Wang; Peng Wang; Hai Li

doi:10.1142/S0218625X09012329

Visualization ex situ of single DNA molecules incubation: A first step for quantitative analysis on multi-site degradation and enzymatic kinetics

Xinyan Wang, Haijun Yang, Huabin Wang, Peng Wang, Hai Li

Research output: Contribution to journal › Article › peer-review

Abstract

Herein, we showed a different approach to directly single-molecule level visualization of the degradation of DNA in vitro tests using DNase I incubation based on high-resolution AFM imaging ex situ with fine relocation nanotechnology. A method of nanomanipulation termed as "modified dynamic molecular combing" (MDMC) was used to pattern DNA samples for further degradation enzymatic kinetics. This strategy is potentially able to quantitatively address the mechanical-induced kinetic profiles of multi-site degradation of individual DNA molecules with very stable tension strong immobilization on a surface discover the mechanochemistry.

Original language	English
Pages (from-to)	79-85
Number of pages	7
Journal	Surface Review and Letters
Volume	16
Issue number	1
DOIs	https://doi.org/10.1142/S0218625X09012329
State	Published - Feb 2009
Externally published	Yes

Keywords

Atomic force microscopy
DNase I
Degradation
Ex situ
Incubation
Modified dynamic molecular combing
Visualization

Access to Document

10.1142/S0218625X09012329

Cite this

@article{30b5ec7f612b49668803b064c5fcb302,

title = "Visualization ex situ of single DNA molecules incubation: A first step for quantitative analysis on multi-site degradation and enzymatic kinetics",

abstract = "Herein, we showed a different approach to directly single-molecule level visualization of the degradation of DNA in vitro tests using DNase I incubation based on high-resolution AFM imaging ex situ with fine relocation nanotechnology. A method of nanomanipulation termed as {"}modified dynamic molecular combing{"} (MDMC) was used to pattern DNA samples for further degradation enzymatic kinetics. This strategy is potentially able to quantitatively address the mechanical-induced kinetic profiles of multi-site degradation of individual DNA molecules with very stable tension strong immobilization on a surface discover the mechanochemistry.",

keywords = "Atomic force microscopy, DNase I, Degradation, Ex situ, Incubation, Modified dynamic molecular combing, Visualization",

author = "Xinyan Wang and Haijun Yang and Huabin Wang and Peng Wang and Hai Li",

note = "Funding Information: We thank Prof. Jun Hu and Prof. Yi Zhang for their helpful assistance with technical supports and very useful discussions. This work was supported by the Grants from the National NSFC (Nos. 10335070 and 10674147), the CAS (Nos. KJCX2-YW-H06 and",

year = "2009",

month = feb,

doi = "10.1142/S0218625X09012329",

language = "English",

volume = "16",

pages = "79--85",

journal = "Surface Review and Letters",

issn = "0218-625X",

publisher = "World Scientific Publishing Co. Pte Ltd",

number = "1",

}

TY - JOUR

T1 - Visualization ex situ of single DNA molecules incubation

T2 - A first step for quantitative analysis on multi-site degradation and enzymatic kinetics

AU - Wang, Xinyan

AU - Yang, Haijun

AU - Wang, Huabin

AU - Wang, Peng

AU - Li, Hai

N1 - Funding Information: We thank Prof. Jun Hu and Prof. Yi Zhang for their helpful assistance with technical supports and very useful discussions. This work was supported by the Grants from the National NSFC (Nos. 10335070 and 10674147), the CAS (Nos. KJCX2-YW-H06 and

PY - 2009/2

Y1 - 2009/2

N2 - Herein, we showed a different approach to directly single-molecule level visualization of the degradation of DNA in vitro tests using DNase I incubation based on high-resolution AFM imaging ex situ with fine relocation nanotechnology. A method of nanomanipulation termed as "modified dynamic molecular combing" (MDMC) was used to pattern DNA samples for further degradation enzymatic kinetics. This strategy is potentially able to quantitatively address the mechanical-induced kinetic profiles of multi-site degradation of individual DNA molecules with very stable tension strong immobilization on a surface discover the mechanochemistry.

AB - Herein, we showed a different approach to directly single-molecule level visualization of the degradation of DNA in vitro tests using DNase I incubation based on high-resolution AFM imaging ex situ with fine relocation nanotechnology. A method of nanomanipulation termed as "modified dynamic molecular combing" (MDMC) was used to pattern DNA samples for further degradation enzymatic kinetics. This strategy is potentially able to quantitatively address the mechanical-induced kinetic profiles of multi-site degradation of individual DNA molecules with very stable tension strong immobilization on a surface discover the mechanochemistry.

KW - Atomic force microscopy

KW - DNase I

KW - Degradation

KW - Ex situ

KW - Incubation

KW - Modified dynamic molecular combing

KW - Visualization

UR - http://www.scopus.com/inward/record.url?scp=65249176797&partnerID=8YFLogxK

U2 - 10.1142/S0218625X09012329

DO - 10.1142/S0218625X09012329

M3 - Article

AN - SCOPUS:65249176797

SN - 0218-625X

VL - 16

SP - 79

EP - 85

JO - Surface Review and Letters

JF - Surface Review and Letters

IS - 1

ER -

Visualization ex situ of single DNA molecules incubation: A first step for quantitative analysis on multi-site degradation and enzymatic kinetics

Abstract

Keywords

Access to Document

Fingerprint

Cite this