Viral expression of epigenome editing tools in rodent brain using stereotaxic surgery techniques

Peter J. Hamilton, Carissa J. Lim, Eric J. Nestler, Elizabeth A. Heller

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

9 Scopus citations

Abstract

Delivery of molecular tools for targeted epigenome editing in rodent brain can be facilitated by the use of viral vector-mediated gene transfer coupled with stereotaxic surgery techniques. Here, we describe the surgical protocol utilized by our group, which is optimized for herpes simplex virus (HSV)-mediated delivery into mouse brain. The protocol outlined herein could also be applied for delivery of adeno-associated viruses (AAV) or lentiviruses in both mice and rats. This method allows for efficient viral transgene expression and subsequent epigenome editing in rodent brain with excellent spatiotemporal control. Nearly any brain region of interest can be targeted in rodents at every stage of postnatal life. Owing to the versatility, reproducibility, and utility of this technique, it is an important method for any laboratory interested in studying the cellular, circuit, and behavioral consequences of in vivo neuroepigenome editing.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages205-214
Number of pages10
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1767
ISSN (Print)1064-3745

Keywords

  • Neuroepigenome editing
  • Rodent brain
  • Stereotaxic surgery
  • Virus-mediated gene transfer

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