Viability and functional activity of cryopreserved mononuclear cells

Adriana Weinberg, Li Zhang, Darby Brown, Alejo Erice, Bruce Polsky, Martin S. Hirsch, Susan Owens, Karan Lamb

Research output: Contribution to journalArticlepeer-review

70 Scopus citations

Abstract

Factors that influence viability and function of cryopreserved peripheral blood mononuclear cells (PBMC) were identified on 54 samples from 27 AIDS Clinical Trial Units. PBMC viability ranged from 1 to 96% with a median of 70%, was higher in laboratories with experienced staff, and was not significantly associated with CD4 cell number. Function of cryopreserved PBMC, measured by lymphocyte proliferation, was associated with viability. Preparations with viability greater than or equal to 70% had consistent proliferative responses and were suitable for functional analyses.

Original languageEnglish
Pages (from-to)714-716
Number of pages3
JournalClinical and Diagnostic Laboratory Immunology
Volume7
Issue number4
DOIs
StatePublished - Jul 2000
Externally publishedYes

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