Viability and functional activity of cryopreserved mononuclear cells

Adriana Weinberg; Li Zhang; Darby Brown; Alejo Erice; Bruce Polsky; Martin S. Hirsch; Susan Owens; Karan Lamb

doi:10.1128/CDLI.7.4.714-716.2000

Viability and functional activity of cryopreserved mononuclear cells

Adriana Weinberg, Li Zhang, Darby Brown, Alejo Erice, Bruce Polsky, Martin S. Hirsch, Susan Owens, Karan Lamb

Research output: Contribution to journal › Article › peer-review

79 Scopus citations

Abstract

Factors that influence viability and function of cryopreserved peripheral blood mononuclear cells (PBMC) were identified on 54 samples from 27 AIDS Clinical Trial Units. PBMC viability ranged from 1 to 96% with a median of 70%, was higher in laboratories with experienced staff, and was not significantly associated with CD4 cell number. Function of cryopreserved PBMC, measured by lymphocyte proliferation, was associated with viability. Preparations with viability greater than or equal to 70% had consistent proliferative responses and were suitable for functional analyses.

Original language	English
Pages (from-to)	714-716
Number of pages	3
Journal	Clinical and Diagnostic Laboratory Immunology
Volume	7
Issue number	4
DOIs	https://doi.org/10.1128/CDLI.7.4.714-716.2000
State	Published - Jul 2000
Externally published	Yes

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10.1128/CDLI.7.4.714-716.2000

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AU - Owens, Susan

AU - Lamb, Karan

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Viability and functional activity of cryopreserved mononuclear cells

Abstract

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