Vascular endothelial growth factor is increased in aqueous humor of glaucomatous eyes

Dan Ning Hu, Robert Ritch, Jeffrey Liebmann, Yizhi Liu, Bin Cheng, Mark S. Hu

Research output: Contribution to journalArticlepeer-review

99 Scopus citations


Purpose: To assess the concentrations of vascular endothelial growth factor (VEGF) in aqueous humor in eyes with and without glaucoma. Methods: Concentrations of VEGF were measured using a sandwich ELISA kit in aqueous humor aspirates taken during anterior segment surgery from 87 patients, of whom 54 had glaucoma (27 primary open-angle glaucoma, 8 angle-closure glaucoma, 16 exfoliative glaucoma) and 33 had cataract only. Results: Vascular endothelial growth factor was detected in all samples. The concentration in eyes with cataract only without glaucoma was 102.4 ± 29.7 pg/mL (mean ± SD), which was significantly lower than that from eyes with glaucoma (146.7 ± 51.8 pg/mL). There were no significant differences between primary open-angle glaucoma (140.4 ± 51.0 pg/mL), angle-closure glaucoma (142.8 ± 40.2 pg/mL), and exfoliative glaucoma (158.6 ± 58.9 pg/mL). An unusually high VEGF concentration was detected in one eye with neovascular glaucoma (759 pg/mL) and two eyes with uveitic glaucoma (322 pg/mL). No effect of age, gender, or previous history of medical, laser, or surgical treatment of the aqueous humor VEGF concentration could be detected (P > 0.05). Aqueous humor and plasma VEGF concentrations were measured and compared in 46 patients. The aqueous humor VEGF concentration (144.2 ± 107.9 pg/mL) was significantly higher (P < 0.01) than the plasma concentration (79.2 ± 46.1 pg/mL). No significant correlation was found between aqueous humor and plasma VEGF concentrations. Conclusion: Aqueous VEGF concentration is increased in eyes with glaucoma.

Original languageEnglish
Pages (from-to)406-410
Number of pages5
JournalJournal of Glaucoma
Issue number5
StatePublished - Oct 2002
Externally publishedYes


  • Aqueous humor
  • Exfoliation
  • Glaucoma
  • Pseudoexfoliation
  • VEGF
  • Vascular endothelial growth factor


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