V-Fps-responsiveness in the Egr-1 promoter is mediated by serum response elements

Konstantina Alexandropoulos, Sajjad A. Qureshi, Myunghi Rim, Vikas P. Sukhatme, David A. Foster

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Egr-1, a mitogen-responsive transcription factor, is rapidly induced by v-Fps in the absence of protein synthesis. Thus, Egr-1 is a primary response to the protein-tyrosine kinase activity of v-Fps. To determine the v-Fps-responsive elements in the Egr-1 promoter, deletion mutants of the Egr-1 promoter were used in transient expression assays. A v-Fps expression vector was cotransfected into NIH 3T3 cells with chloramphenicol acetyl transferase (CAT) gene expression vectors under the control of the Egr-1 promoter or the Egr-1 promoter containing various deletions. Responsiveness to v-Fps was restricted to a region that contained repeated CC(A/T)6GG sequences, known as CArG boxes. CArG boxes form the core of serum response element (SREs). v-Fps induced Egr-1 promoter activation was lost by sequential removal of four tandemly repeated SREs. This region, containing four SREs, was found to be sufficient for maximal Egr-1 induction by v-Fps when placed upstream from a heterologous promoter. Individual SREs from this region were able to respond to v-Fps, however, the activation of the individual SREs was lower than that observed for the clustered SREs. These data suggest that v-Fps-responsiveness in the Egr-1 promoter is mediated by SREs.

Original languageEnglish
Pages (from-to)2355-2359
Number of pages5
JournalNucleic Acids Research
Volume20
Issue number9
DOIs
StatePublished - 11 May 1992
Externally publishedYes

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