TY - JOUR
T1 - Using the Culex pipiens sperm proteome to identify elements essential for mosquito reproduction
AU - Thaler, Catherine D.
AU - Carstens, Kaira
AU - Martinez, Gabrielle
AU - Stephens, Kimberly
AU - Cardullo, Richard A.
N1 - Publisher Copyright:
Copyright: © 2023 Thaler et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2023/2
Y1 - 2023/2
N2 - Mature sperm from Culex pipiens were isolated and analyzed by mass spectrometry to generate a mature sperm proteome dataset. In this study, we highlight subsets of proteins related to flagellar structure and sperm motility and compare the identified protein components to previous studies examining essential functions of sperm. The proteome includes 1700 unique protein IDs, including a number of uncharacterized proteins. Here we discuss those proteins that may contribute to the unusual structure of the Culex sperm flagellum, as well as potential regulators of calcium mobilization and phosphorylation pathways that regulate motility. This database will prove useful for understanding the mechanisms that activate and maintain sperm motility as well as identify potential molecular targets for mosquito population control.
AB - Mature sperm from Culex pipiens were isolated and analyzed by mass spectrometry to generate a mature sperm proteome dataset. In this study, we highlight subsets of proteins related to flagellar structure and sperm motility and compare the identified protein components to previous studies examining essential functions of sperm. The proteome includes 1700 unique protein IDs, including a number of uncharacterized proteins. Here we discuss those proteins that may contribute to the unusual structure of the Culex sperm flagellum, as well as potential regulators of calcium mobilization and phosphorylation pathways that regulate motility. This database will prove useful for understanding the mechanisms that activate and maintain sperm motility as well as identify potential molecular targets for mosquito population control.
UR - https://www.scopus.com/pages/publications/85148306811
U2 - 10.1371/journal.pone.0280013
DO - 10.1371/journal.pone.0280013
M3 - Article
C2 - 36795667
AN - SCOPUS:85148306811
SN - 1932-6203
VL - 18
JO - PLoS ONE
JF - PLoS ONE
IS - 2 February
M1 - e0280013
ER -