Use of real-time PCR and molecular beacons to detect virus replication in human immunodeficiency virus type 1-infected individuals on prolonged effective antiretroviral therapy

S. R. Lewin, M. Vesanen, L. Kostrikis, A. Hurley, M. Duran, L. Zhang, D. D. Ho, M. Markowitz, U. E. Gibson, C. A. Heid

Research output: Contribution to journalArticlepeer-review

213 Scopus citations

Abstract

We have designed a novel, precise, and sensitive assay to measure unspliced (US) human immunodeficiency virus type 1 (HIV-1) mRNA in peripheral blood mononuclear cells of HIV-1-infected individuals by using real-time PCR and molecular beacons. Individuals were classified as either well suppressed (WS) or partially suppressed, based on longitudinal measurements of plasma HIV-1 RNA. The proportion of individuals with US mRNA undetectable over time was significantly higher among WS individuals; however, 30% of WS subjects still had detectable US mRNA after 24 months of effective antiviral therapy.

Original languageEnglish
Pages (from-to)6099-6103
Number of pages5
JournalJournal of Virology
Volume73
Issue number7
DOIs
StatePublished - 1999
Externally publishedYes

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