Use of aequorin-based indicators for monitoring Ca2+ in acidic organelles

M. T. Alonso, P. Torres-Vidal, B. Calvo, C. Rodriguez, A. Delrio-Lorenzo, J. Rojo-Ruiz, J. Garcia-Sancho, S. Patel

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Over the last years, there is accumulating evidence that acidic organelles can accumulate and release Ca2+ upon cell activation. Hence, reliable recording of Ca2+ dynamics in these compartments is essential for understanding the physiopathological aspects of acidic organelles. Genetically encoded Ca2+ indicators (GECIs) are valuable tools to monitor Ca2+ in specific locations, although their use in acidic compartments is challenging due to the pH sensitivity of most available fluorescent GECIs. By contrast, bioluminescent GECIs have a combination of features (marginal pH sensitivity, low background, no phototoxicity, no photobleaching, high dynamic range and tunable affinity) that render them advantageous to achieve an enhanced signal-to-noise ratio in acidic compartments. This article reviews the use of bioluminescent aequorin-based GECIs targeted to acidic compartments. A need for more measurements in highly acidic compartments is identified.

Original languageEnglish
Article number119481
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1870
Issue number6
DOIs
StatePublished - Aug 2023
Externally publishedYes

Keywords

  • Acidic
  • Ca signalling
  • Endosome
  • GECI
  • Genetically encoded calcium indicator
  • Luminescence
  • Lysosome
  • Organelle
  • Secretory vesicle
  • Subcellular Ca

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