Use of a short fragment of the C-terminal E gene for detection and characterization of two new lineages of dengue virus 1 in India

C. Domingo, G. Palacios, O. Jabado, N. Reyes, M. Niedrig, J. Gascón, M. Cabrerizo, W. I. Lipkin, A. Tenorio

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

Here we propose the use of a 216-nucleotide fragment located in the carboxyl terminus of the E gene (E-COOH) and a pairwise-based comparison method for genotyping of dengue virus 1 (DENV-1) strains. We have applied this method to the detection and characterization of DENV-1 in serum samples from travelers returning from the tropics. The results obtained with the typing system correlate with the results obtained by comparison of the sequences of the entire E gene of the strains. The approach demonstrates utility in plotting the distribution and circulation of different genotypes of DENV-1 and also suggests the presence of two new clades of Indian strains. The integration of the method with an online database and a typing characterization tool enhances its strength. Additionally, the analysis of the complete E gene of DENV-1 strains suggested the occurrence of a nondescribed recombination event in the China GD23-95 strain. We propose the use of this methodology as a tool for real-time epidemiological surveillance of dengue virus infections and their pathogenesis.

Original languageEnglish
Pages (from-to)1519-1529
Number of pages11
JournalJournal of Clinical Microbiology
Volume44
Issue number4
DOIs
StatePublished - Apr 2006
Externally publishedYes

Fingerprint

Dive into the research topics of 'Use of a short fragment of the C-terminal E gene for detection and characterization of two new lineages of dengue virus 1 in India'. Together they form a unique fingerprint.

Cite this