TY - JOUR
T1 - Upregulation of galanin binding sites and GalR1 mRNA levels in the mouse locus coeruleus following chronic morphine treatments and precipitated morphine withdrawal
AU - Zachariou, Venetia
AU - Thome, Johannes
AU - Parikh, Kavita
AU - Picciotto, Marina R.
N1 - Funding Information:
The authors thank Drs. M. Zoli, R. Duman, and E. Nestler for helpful conversations about the work. This work was supported by Grant #DA08227 from the National Institutes of Health, a grant from the Donaghue Foundation, and The Christiane Brooks Johnson Foundation.
PY - 2000/8
Y1 - 2000/8
N2 - The neuropeptide galanin and its receptors are expressed in the locus coeruleus (LC), a brain area associated with drug dependence and withdrawal. Although galanin peptide mRNA levels do not change during withdrawal, it is not known whether galanin receptor levels are regulated following opiate withdrawal. This study demonstrates that galanin binding in the LC is upregulated by chronic-intermittent morphine administration or by precipitated withdrawal, but not by acute morphine treatment, suggesting that increased activity in the LC may be able to regulate galanin binding sites. Moreover, the increase in galanin binding sites seems to be caused by increased transcription or stabilization of the galanin receptor 1 (GalR1) gene, because there is a dramatic increase in mRNA levels following withdrawal in the LC. It is, therefore, possible that the increase in GalR1 could be an adaptive mechanism that leads to regulation of cAMP levels and possibly firing rate of LC neurons. Copyright (C) 2000 American College of Neuropsychopharmacology.
AB - The neuropeptide galanin and its receptors are expressed in the locus coeruleus (LC), a brain area associated with drug dependence and withdrawal. Although galanin peptide mRNA levels do not change during withdrawal, it is not known whether galanin receptor levels are regulated following opiate withdrawal. This study demonstrates that galanin binding in the LC is upregulated by chronic-intermittent morphine administration or by precipitated withdrawal, but not by acute morphine treatment, suggesting that increased activity in the LC may be able to regulate galanin binding sites. Moreover, the increase in galanin binding sites seems to be caused by increased transcription or stabilization of the galanin receptor 1 (GalR1) gene, because there is a dramatic increase in mRNA levels following withdrawal in the LC. It is, therefore, possible that the increase in GalR1 could be an adaptive mechanism that leads to regulation of cAMP levels and possibly firing rate of LC neurons. Copyright (C) 2000 American College of Neuropsychopharmacology.
KW - Drug dependence
KW - Galanin receptors
KW - Locus coeruleus, In situ hybridization
KW - Mouse
KW - Receptor autoradiography
UR - https://www.scopus.com/pages/publications/0034086917
U2 - 10.1016/S0893-133X(00)00094-4
DO - 10.1016/S0893-133X(00)00094-4
M3 - Article
C2 - 10882839
AN - SCOPUS:0034086917
SN - 0893-133X
VL - 23
SP - 127
EP - 137
JO - Neuropsychopharmacology
JF - Neuropsychopharmacology
IS - 2
ER -