TY - JOUR
T1 - Up-regulated p75(NTR) neurotrophin receptor on glial cells in MS plaques
AU - Dowling, Peter
AU - Ming, X.
AU - Raval, S.
AU - Husar, W.
AU - Casaccia-Bonnefil, P.
AU - Chao, M.
AU - Cook, S.
AU - Blumberg, B.
PY - 1999/11/10
Y1 - 1999/11/10
N2 - Objective: To investigate the expression of the neurotrophin receptor p75(NTR) on glial cells within MS plaques. Background: In recent studies on the pathogenesis of MS white matter plaques, we found large populations of inflammatory and resident glial cells, including oligodendrocytes undergoing cell death, and identified increased expression of Fas receptor and ligand death pathway signaling molecules on the same glial cell types. In another study, the p75(NTR) was shown to induce apoptotic death of maturing oligodendrocytes when exposed to NGF in vitro. Methods: We used immunohistochemistry and in situ reverse-transcription PCR to detect p75(NTR) expression on inflammatory and resident glial cells in MS plaques and used TUNEL staining for fragmented DNA to detect cell death. Results: Upregulated p75(NTR) messenger RNA and protein were demonstrated in both oligodendrocytes and microglia/macrophages in MS plaques but not in control white matter. However, only a fraction of p75(NTR) expressing oligodendrocytes was also stained by TUNEL. Conclusions: Glial cell expression of p75(NTR) receptor is up-regulated during MS plaque formation. The exact role of this receptor in glial cell death and/or survival in MS remains to be elucidated.
AB - Objective: To investigate the expression of the neurotrophin receptor p75(NTR) on glial cells within MS plaques. Background: In recent studies on the pathogenesis of MS white matter plaques, we found large populations of inflammatory and resident glial cells, including oligodendrocytes undergoing cell death, and identified increased expression of Fas receptor and ligand death pathway signaling molecules on the same glial cell types. In another study, the p75(NTR) was shown to induce apoptotic death of maturing oligodendrocytes when exposed to NGF in vitro. Methods: We used immunohistochemistry and in situ reverse-transcription PCR to detect p75(NTR) expression on inflammatory and resident glial cells in MS plaques and used TUNEL staining for fragmented DNA to detect cell death. Results: Upregulated p75(NTR) messenger RNA and protein were demonstrated in both oligodendrocytes and microglia/macrophages in MS plaques but not in control white matter. However, only a fraction of p75(NTR) expressing oligodendrocytes was also stained by TUNEL. Conclusions: Glial cell expression of p75(NTR) receptor is up-regulated during MS plaque formation. The exact role of this receptor in glial cell death and/or survival in MS remains to be elucidated.
KW - Glial cells
KW - MS
KW - Neurotrophin receptor p75(NTR)
KW - White matter plaques
UR - http://www.scopus.com/inward/record.url?scp=0033544034&partnerID=8YFLogxK
U2 - 10.1212/wnl.53.8.1676
DO - 10.1212/wnl.53.8.1676
M3 - Article
C2 - 10563612
AN - SCOPUS:0033544034
SN - 0028-3878
VL - 53
SP - 1676
EP - 1682
JO - Neurology
JF - Neurology
IS - 8
ER -