Unusual DNA structures at the integration site of an HIV provirus

Miguel A. Gama Sosa; Jessica C. Hall; Karen E. Schneider; Gregory C. Lukaszewicz; Ruth M. Ruprecht

doi:10.1016/0006-291X(89)91571-4

Unusual DNA structures at the integration site of an HIV provirus

Miguel A. Gama Sosa, Jessica C. Hall, Karen E. Schneider, Gregory C. Lukaszewicz, Ruth M. Ruprecht

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Supercoiled pHXBc2 DNA (containing the genome of the human immunodeficiency virus type 1 and human sequences) migrated more slowly than linear DNA in native and ethidium bromide agarose gel electrophoresis at 4.5 volts/cm, suggesting the presence of unusual DNA structures. S1 nuclease analysis of pHXBc2 revealed two S1 hypersensitive sites. Site I was located within a 25 bp direct repeat in host DNA 0.6 kB upstream from the 5′ LTR. Site II was mapped 0.2 kB upstream from the vif gene start site. Sequence analysis showed that Site I sequences could assume different unusual DNA structures, whereas sequences at Site II could assume either slipped or H-DNA forms. Unusual DNA structures in host DNA may be associated with active chromatin regions and may favor proviral integration.

Original language	English
Pages (from-to)	134-142
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	161
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(89)91571-4
State	Published - 30 May 1989
Externally published	Yes

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title = "Unusual DNA structures at the integration site of an HIV provirus",

abstract = "Supercoiled pHXBc2 DNA (containing the genome of the human immunodeficiency virus type 1 and human sequences) migrated more slowly than linear DNA in native and ethidium bromide agarose gel electrophoresis at 4.5 volts/cm, suggesting the presence of unusual DNA structures. S1 nuclease analysis of pHXBc2 revealed two S1 hypersensitive sites. Site I was located within a 25 bp direct repeat in host DNA 0.6 kB upstream from the 5′ LTR. Site II was mapped 0.2 kB upstream from the vif gene start site. Sequence analysis showed that Site I sequences could assume different unusual DNA structures, whereas sequences at Site II could assume either slipped or H-DNA forms. Unusual DNA structures in host DNA may be associated with active chromatin regions and may favor proviral integration.",

author = "{Gama Sosa}, {Miguel A.} and Hall, {Jessica C.} and Schneider, {Karen E.} and Lukaszewicz, {Gregory C.} and Ruprecht, {Ruth M.}",

note = "Funding Information: The authors wish to thank Dr. Sodroski for the pHXBc2 clone and Rita Antonelli for preparing this manuscript. Supported inpartby grant #DMD-8509459 from the National Science Foundation and contract #FOD-0757 from the National Institute of Health. RMR acknowledges a Science Scholarship of the Mary Ingraham Bunting Institute of Radcliffe College and a Faculty Development Award from the Pharmaceutical Manufacturers Association Foundation.",

year = "1989",

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doi = "10.1016/0006-291X(89)91571-4",

language = "English",

volume = "161",

pages = "134--142",

journal = "Biochemical and Biophysical Research Communications",

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T1 - Unusual DNA structures at the integration site of an HIV provirus

AU - Gama Sosa, Miguel A.

AU - Hall, Jessica C.

AU - Schneider, Karen E.

AU - Lukaszewicz, Gregory C.

AU - Ruprecht, Ruth M.

N1 - Funding Information: The authors wish to thank Dr. Sodroski for the pHXBc2 clone and Rita Antonelli for preparing this manuscript. Supported inpartby grant #DMD-8509459 from the National Science Foundation and contract #FOD-0757 from the National Institute of Health. RMR acknowledges a Science Scholarship of the Mary Ingraham Bunting Institute of Radcliffe College and a Faculty Development Award from the Pharmaceutical Manufacturers Association Foundation.

PY - 1989/5/30

Y1 - 1989/5/30

N2 - Supercoiled pHXBc2 DNA (containing the genome of the human immunodeficiency virus type 1 and human sequences) migrated more slowly than linear DNA in native and ethidium bromide agarose gel electrophoresis at 4.5 volts/cm, suggesting the presence of unusual DNA structures. S1 nuclease analysis of pHXBc2 revealed two S1 hypersensitive sites. Site I was located within a 25 bp direct repeat in host DNA 0.6 kB upstream from the 5′ LTR. Site II was mapped 0.2 kB upstream from the vif gene start site. Sequence analysis showed that Site I sequences could assume different unusual DNA structures, whereas sequences at Site II could assume either slipped or H-DNA forms. Unusual DNA structures in host DNA may be associated with active chromatin regions and may favor proviral integration.

AB - Supercoiled pHXBc2 DNA (containing the genome of the human immunodeficiency virus type 1 and human sequences) migrated more slowly than linear DNA in native and ethidium bromide agarose gel electrophoresis at 4.5 volts/cm, suggesting the presence of unusual DNA structures. S1 nuclease analysis of pHXBc2 revealed two S1 hypersensitive sites. Site I was located within a 25 bp direct repeat in host DNA 0.6 kB upstream from the 5′ LTR. Site II was mapped 0.2 kB upstream from the vif gene start site. Sequence analysis showed that Site I sequences could assume different unusual DNA structures, whereas sequences at Site II could assume either slipped or H-DNA forms. Unusual DNA structures in host DNA may be associated with active chromatin regions and may favor proviral integration.

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SN - 0006-291X

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SP - 134

EP - 142

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

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Unusual DNA structures at the integration site of an HIV provirus

Abstract

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