Uniform detection of HIV-1 in alveolar macrophages of pediatric but not adult AIDS patients

T. E. Pearce; M. Nowakowski; E. Eden; Z. B. Huang; P. Steiner; M. Shahabuddin; M. J. Potash; D. J. Volsky

doi:10.1002/jlb.53.6.722

Uniform detection of HIV-1 in alveolar macrophages of pediatric but not adult AIDS patients

T. E. Pearce, M. Nowakowski, E. Eden, Z. B. Huang, P. Steiner, M. Shahabuddin, M. J. Potash, D. J. Volsky

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Manifestations of pulmonary disease in pediatric AIDS patients differ from those in adults. To evaluate whether differences in the frequency of alveolar macrophage (AM) infection with human immunodeficiency virus type 1 (HIV-1) could account for these clinical distinctions, we undertook a comparative analysis of HIV-1 DNA in AMs from pediatric and adult AIDS patients by enzymatic amplification. A higher frequency of viral DNA detection in pediatric cases (100%) compared with adults (67%) was observed. The sensitivity of detection was 1 viral DNA copy per 4000 AMs; matched peripheral blood mononuclear cells from six of seven pediatric and eight of nine adult patients tested HIV-1 DNA positive. Adult but not pediatric patients exhibited a marked alveolar lymphocytosis, 32% mean lymphocyte count compared with 7.0%, respectively. These results suggest that the burden of HIV-1 in the lungs of pediatric AIDS patients is greater than that in adults.

Original language	English
Pages (from-to)	722-726
Number of pages	5
Journal	Journal of Leukocyte Biology
Volume	53
Issue number	6
DOIs	https://doi.org/10.1002/jlb.53.6.722
State	Published - 1993
Externally published	Yes

Keywords

AIDS
HIV-1
alveolar macrophages
pediatric AIDS

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10.1002/jlb.53.6.722

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title = "Uniform detection of HIV-1 in alveolar macrophages of pediatric but not adult AIDS patients",

abstract = "Manifestations of pulmonary disease in pediatric AIDS patients differ from those in adults. To evaluate whether differences in the frequency of alveolar macrophage (AM) infection with human immunodeficiency virus type 1 (HIV-1) could account for these clinical distinctions, we undertook a comparative analysis of HIV-1 DNA in AMs from pediatric and adult AIDS patients by enzymatic amplification. A higher frequency of viral DNA detection in pediatric cases (100%) compared with adults (67%) was observed. The sensitivity of detection was 1 viral DNA copy per 4000 AMs; matched peripheral blood mononuclear cells from six of seven pediatric and eight of nine adult patients tested HIV-1 DNA positive. Adult but not pediatric patients exhibited a marked alveolar lymphocytosis, 32% mean lymphocyte count compared with 7.0%, respectively. These results suggest that the burden of HIV-1 in the lungs of pediatric AIDS patients is greater than that in adults.",

keywords = "AIDS, HIV-1, alveolar macrophages, pediatric AIDS",

author = "Pearce, {T. E.} and M. Nowakowski and E. Eden and Huang, {Z. B.} and P. Steiner and M. Shahabuddin and Potash, {M. J.} and Volsky, {D. J.}",

year = "1993",

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TY - JOUR

T1 - Uniform detection of HIV-1 in alveolar macrophages of pediatric but not adult AIDS patients

AU - Pearce, T. E.

AU - Nowakowski, M.

AU - Eden, E.

AU - Huang, Z. B.

AU - Steiner, P.

AU - Shahabuddin, M.

AU - Potash, M. J.

AU - Volsky, D. J.

PY - 1993

Y1 - 1993

N2 - Manifestations of pulmonary disease in pediatric AIDS patients differ from those in adults. To evaluate whether differences in the frequency of alveolar macrophage (AM) infection with human immunodeficiency virus type 1 (HIV-1) could account for these clinical distinctions, we undertook a comparative analysis of HIV-1 DNA in AMs from pediatric and adult AIDS patients by enzymatic amplification. A higher frequency of viral DNA detection in pediatric cases (100%) compared with adults (67%) was observed. The sensitivity of detection was 1 viral DNA copy per 4000 AMs; matched peripheral blood mononuclear cells from six of seven pediatric and eight of nine adult patients tested HIV-1 DNA positive. Adult but not pediatric patients exhibited a marked alveolar lymphocytosis, 32% mean lymphocyte count compared with 7.0%, respectively. These results suggest that the burden of HIV-1 in the lungs of pediatric AIDS patients is greater than that in adults.

AB - Manifestations of pulmonary disease in pediatric AIDS patients differ from those in adults. To evaluate whether differences in the frequency of alveolar macrophage (AM) infection with human immunodeficiency virus type 1 (HIV-1) could account for these clinical distinctions, we undertook a comparative analysis of HIV-1 DNA in AMs from pediatric and adult AIDS patients by enzymatic amplification. A higher frequency of viral DNA detection in pediatric cases (100%) compared with adults (67%) was observed. The sensitivity of detection was 1 viral DNA copy per 4000 AMs; matched peripheral blood mononuclear cells from six of seven pediatric and eight of nine adult patients tested HIV-1 DNA positive. Adult but not pediatric patients exhibited a marked alveolar lymphocytosis, 32% mean lymphocyte count compared with 7.0%, respectively. These results suggest that the burden of HIV-1 in the lungs of pediatric AIDS patients is greater than that in adults.

KW - AIDS

KW - HIV-1

KW - alveolar macrophages

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Uniform detection of HIV-1 in alveolar macrophages of pediatric but not adult AIDS patients

Abstract

Keywords

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