Uniform detection of HIV-1 in alveolar macrophages of pediatric but not adult AIDS patients

T. E. Pearce, M. Nowakowski, E. Eden, Z. B. Huang, P. Steiner, M. Shahabuddin, M. J. Potash, D. J. Volsky

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14 Scopus citations


Manifestations of pulmonary disease in pediatric AIDS patients differ from those in adults. To evaluate whether differences in the frequency of alveolar macrophage (AM) infection with human immunodeficiency virus type 1 (HIV-1) could account for these clinical distinctions, we undertook a comparative analysis of HIV-1 DNA in AMs from pediatric and adult AIDS patients by enzymatic amplification. A higher frequency of viral DNA detection in pediatric cases (100%) compared with adults (67%) was observed. The sensitivity of detection was 1 viral DNA copy per 4000 AMs; matched peripheral blood mononuclear cells from six of seven pediatric and eight of nine adult patients tested HIV-1 DNA positive. Adult but not pediatric patients exhibited a marked alveolar lymphocytosis, 32% mean lymphocyte count compared with 7.0%, respectively. These results suggest that the burden of HIV-1 in the lungs of pediatric AIDS patients is greater than that in adults.

Original languageEnglish
Pages (from-to)722-726
Number of pages5
JournalJournal of Leukocyte Biology
Issue number6
StatePublished - 1993
Externally publishedYes


  • AIDS
  • HIV-1
  • alveolar macrophages
  • pediatric AIDS


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