Unidirectional electrical pulses for cell alignment in a closed microfluidic chamber

Despina Nelie Loufakis, Zhenning Cao, Sai Ma, David Mittelman, Chang Lu

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

The transport and manipulation of cells in microfluidic structures are often critically required in cellular analysis. Cells typically make consistent movement in a DC electric field in a single direction, due to a combination of their electrophoretic mobility and electroosmotic flow. Here, we demonstrate that mammalian cells focus to the middle of a closed microfluidic chamber under the application of unidirectional DC pulses. Under the pulses, electrochemical reactions take place and create a nonlinear pH gradient, which affects the cell surface charge. Our approach provides a new and simple method for concentrating mammalian cells at the microscale.

Original languageEnglish
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages473-475
Number of pages3
ISBN (Electronic)9780979806476
StatePublished - 2014
Externally publishedYes
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: 26 Oct 201430 Oct 2014

Publication series

Name18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Conference

Conference18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Country/TerritoryUnited States
CitySan Antonio
Period26/10/1430/10/14

Keywords

  • Cell concentration
  • Isoelectric focusing
  • Mammalian cells
  • PH gradient

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