Ultratight crystal packing of a 10 kDa protein

Sergio Trillo-Muyo; Andrius Jasilionis; Marcin J. Domagalski; Maksymilian Chruszcz; Wladek Minor; Nomeda Kuisiene; Joan L. Arolas; Maria Solà; F. Xavier Gomis-Rüth

doi:10.1107/S0907444912050135

Ultratight crystal packing of a 10 kDa protein

Sergio Trillo-Muyo, Andrius Jasilionis, Marcin J. Domagalski, Maksymilian Chruszcz, Wladek Minor, Nomeda Kuisiene, Joan L. Arolas, Maria Solà, F. Xavier Gomis-Rüth

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

While small organic molecules generally crystallize forming tightly packed lattices with little solvent content, proteins form air-sensitive high-solvent-content crystals. Here, the crystallization and full structure analysis of a novel recombinant 10 kDa protein corresponding to the C-terminal domain of a putative U32 peptidase are reported. The orthorhombic crystal contained only 24.5% solvent and is therefore among the most tightly packed protein lattices ever reported.

Original language	English
Pages (from-to)	464-470
Number of pages	7
Journal	Acta Crystallographica Section D: Biological Crystallography
Volume	69
Issue number	3
DOIs	https://doi.org/10.1107/S0907444912050135
State	Published - Mar 2013
Externally published	Yes

Keywords

crystal packing
high resolution
solvent content

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10.1107/S0907444912050135

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title = "Ultratight crystal packing of a 10 kDa protein",

abstract = "While small organic molecules generally crystallize forming tightly packed lattices with little solvent content, proteins form air-sensitive high-solvent-content crystals. Here, the crystallization and full structure analysis of a novel recombinant 10 kDa protein corresponding to the C-terminal domain of a putative U32 peptidase are reported. The orthorhombic crystal contained only 24.5% solvent and is therefore among the most tightly packed protein lattices ever reported.",

keywords = "crystal packing, high resolution, solvent content",

author = "Sergio Trillo-Muyo and Andrius Jasilionis and Domagalski, {Marcin J.} and Maksymilian Chruszcz and Wladek Minor and Nomeda Kuisiene and Arolas, {Joan L.} and Maria Sol{\`a} and Gomis-R{\"u}th, {F. Xavier}",

year = "2013",

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doi = "10.1107/S0907444912050135",

language = "English",

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journal = "Acta Crystallographica Section D: Biological Crystallography",

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T1 - Ultratight crystal packing of a 10 kDa protein

AU - Trillo-Muyo, Sergio

AU - Jasilionis, Andrius

AU - Domagalski, Marcin J.

AU - Chruszcz, Maksymilian

AU - Minor, Wladek

AU - Kuisiene, Nomeda

AU - Arolas, Joan L.

AU - Solà, Maria

AU - Gomis-Rüth, F. Xavier

PY - 2013/3

Y1 - 2013/3

N2 - While small organic molecules generally crystallize forming tightly packed lattices with little solvent content, proteins form air-sensitive high-solvent-content crystals. Here, the crystallization and full structure analysis of a novel recombinant 10 kDa protein corresponding to the C-terminal domain of a putative U32 peptidase are reported. The orthorhombic crystal contained only 24.5% solvent and is therefore among the most tightly packed protein lattices ever reported.

AB - While small organic molecules generally crystallize forming tightly packed lattices with little solvent content, proteins form air-sensitive high-solvent-content crystals. Here, the crystallization and full structure analysis of a novel recombinant 10 kDa protein corresponding to the C-terminal domain of a putative U32 peptidase are reported. The orthorhombic crystal contained only 24.5% solvent and is therefore among the most tightly packed protein lattices ever reported.

KW - crystal packing

KW - high resolution

KW - solvent content

UR - http://www.scopus.com/inward/record.url?scp=84875442869&partnerID=8YFLogxK

U2 - 10.1107/S0907444912050135

DO - 10.1107/S0907444912050135

M3 - Article

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AN - SCOPUS:84875442869

SN - 0907-4449

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SP - 464

EP - 470

JO - Acta Crystallographica Section D: Biological Crystallography

JF - Acta Crystallographica Section D: Biological Crystallography

IS - 3

ER -

Ultratight crystal packing of a 10 kDa protein

Abstract

Keywords

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