TY - JOUR
T1 - Ultraperformance liquid chromatography with electrospray ionization ion trap mass spectrometry for chondroitin disaccharide analysis
AU - Solakyildirim, Kemal
AU - Zhang, Zhenqing
AU - Linhardt, Robert J.
PY - 2010/2/1
Y1 - 2010/2/1
N2 - Chondroitin sulfate (CS) has an important role in cell division, in the central nervous system, and in joint-related pathologies such as osteoarthritis. Due to the complex chemical structure and biological importance of CS, simple, sensitive, high resolution, and robust analytical methods are needed for the analysis of CS disaccharides and oligosaccharides. An ion-pairing, reversed-phase, ultraperformance liquid chromatography (IPRP-UPLC) separation, coupled to electrospray ionization mass spectrometry with an ion trap mass analyzer, was applied for the analyses of CS-derived disaccharides. UPLC separation technology uses small particle diameter, short column length, and elevated column temperature to obtain high resolution and sensitivity. Hexylamine (15 mM) was selected as the optimal ion-pairing reagent.
AB - Chondroitin sulfate (CS) has an important role in cell division, in the central nervous system, and in joint-related pathologies such as osteoarthritis. Due to the complex chemical structure and biological importance of CS, simple, sensitive, high resolution, and robust analytical methods are needed for the analysis of CS disaccharides and oligosaccharides. An ion-pairing, reversed-phase, ultraperformance liquid chromatography (IPRP-UPLC) separation, coupled to electrospray ionization mass spectrometry with an ion trap mass analyzer, was applied for the analyses of CS-derived disaccharides. UPLC separation technology uses small particle diameter, short column length, and elevated column temperature to obtain high resolution and sensitivity. Hexylamine (15 mM) was selected as the optimal ion-pairing reagent.
KW - Chondroitin sulfate
KW - Dermatan sulfate
KW - Disaccharides
KW - Glycosaminoglycans
KW - Ion-pairing
KW - LC-MS
KW - Reversed phase
UR - http://www.scopus.com/inward/record.url?scp=71749085244&partnerID=8YFLogxK
U2 - 10.1016/j.ab.2009.09.031
DO - 10.1016/j.ab.2009.09.031
M3 - Article
C2 - 19769936
AN - SCOPUS:71749085244
SN - 0003-2697
VL - 397
SP - 24
EP - 28
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -