TY - JOUR
T1 - Two-way regulation between cells and aligned collagen fibrils
T2 - Local 3D matrix formation and accelerated neural differentiation of human decidua parietalis placental stem cells
AU - Li, Wen
AU - Zhu, Bofan
AU - Strakova, Zuzana
AU - Wang, Rong
N1 - Funding Information:
This research was supported by NIH ( R01 NS047719 ).
PY - 2014/8/8
Y1 - 2014/8/8
N2 - It has been well established that an aligned matrix provides structural and signaling cues to guide cell polarization and cell fate decision. However, the modulation role of cells in matrix remodeling and the feedforward effect on stem cell differentiation have not been studied extensively. In this study, we report on the concerted changes of human decidua parietalis placental stem cells (hdpPSCs) and the highly ordered collagen fibril matrix in response to cell-matrix interaction. With high-resolution imaging, we found the hdpPSCs interacted with the matrix by deforming the cell shape, harvesting the nearby collagen fibrils, and reorganizing the fibrils around the cell body to transform a 2D matrix to a localized 3D matrix. Such a unique 3D matrix prompted high expression of β-1 integrin around the cell body that mediates and facilitates the stem cell differentiation toward neural cells. The study offers insights into the coordinated, dynamic changes at the cell-matrix interface and elucidates cell modulation of its matrix to establish structural and biochemical cues for effective cell growth and differentiation.
AB - It has been well established that an aligned matrix provides structural and signaling cues to guide cell polarization and cell fate decision. However, the modulation role of cells in matrix remodeling and the feedforward effect on stem cell differentiation have not been studied extensively. In this study, we report on the concerted changes of human decidua parietalis placental stem cells (hdpPSCs) and the highly ordered collagen fibril matrix in response to cell-matrix interaction. With high-resolution imaging, we found the hdpPSCs interacted with the matrix by deforming the cell shape, harvesting the nearby collagen fibrils, and reorganizing the fibrils around the cell body to transform a 2D matrix to a localized 3D matrix. Such a unique 3D matrix prompted high expression of β-1 integrin around the cell body that mediates and facilitates the stem cell differentiation toward neural cells. The study offers insights into the coordinated, dynamic changes at the cell-matrix interface and elucidates cell modulation of its matrix to establish structural and biochemical cues for effective cell growth and differentiation.
KW - 3D matrix
KW - Atomic force microscopy (AFM)
KW - Cell polarization
KW - Collagen
KW - Fibril alignment
KW - Neural differentiation
UR - https://www.scopus.com/pages/publications/84906085903
U2 - 10.1016/j.bbrc.2014.06.136
DO - 10.1016/j.bbrc.2014.06.136
M3 - Article
C2 - 25003322
AN - SCOPUS:84906085903
SN - 0006-291X
VL - 450
SP - 1377
EP - 1382
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -