Two-Photon, Ratiometric, Quantitative Fluorescent Probe Reveals Fluctuation of Peroxynitrite Regulated by Arginase 1

Shiyu Chen; Beyza Vurusaner; Stephanie Pena; Chu T. Thu; Lara K. Mahal; Edward A. Fisher; James W. Canary

doi:10.1021/acs.analchem.1c00911

Two-Photon, Ratiometric, Quantitative Fluorescent Probe Reveals Fluctuation of Peroxynitrite Regulated by Arginase 1

Shiyu Chen, Beyza Vurusaner, Stephanie Pena, Chu T. Thu, Lara K. Mahal, Edward A. Fisher, James W. Canary

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Peroxynitrite, a transient reactive oxygen species (ROS), is believed to play a deleterious role in physiological processes. Herein, we report a two-photon ratiometric fluorescent probe that selectively reacts with peroxynitrite yielding a >200-fold change upon reaction. The probe effectively visualized fluctuations in peroxynitrite generation by arginase 1 in vivo and in vitro. This provides evidence that arginase 1 is a critical regulator of peroxynitrite.

Original language	English
Pages (from-to)	10090-10098
Number of pages	9
Journal	Analytical Chemistry
Volume	93
Issue number	29
DOIs	https://doi.org/10.1021/acs.analchem.1c00911
State	Published - 27 Jul 2021
Externally published	Yes

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abstract = "Peroxynitrite, a transient reactive oxygen species (ROS), is believed to play a deleterious role in physiological processes. Herein, we report a two-photon ratiometric fluorescent probe that selectively reacts with peroxynitrite yielding a >200-fold change upon reaction. The probe effectively visualized fluctuations in peroxynitrite generation by arginase 1 in vivo and in vitro. This provides evidence that arginase 1 is a critical regulator of peroxynitrite.",

author = "Shiyu Chen and Beyza Vurusaner and Stephanie Pena and Thu, {Chu T.} and Mahal, {Lara K.} and Fisher, {Edward A.} and Canary, {James W.}",

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AU - Chen, Shiyu

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AU - Pena, Stephanie

AU - Thu, Chu T.

AU - Mahal, Lara K.

AU - Fisher, Edward A.

AU - Canary, James W.

PY - 2021/7/27

Y1 - 2021/7/27

N2 - Peroxynitrite, a transient reactive oxygen species (ROS), is believed to play a deleterious role in physiological processes. Herein, we report a two-photon ratiometric fluorescent probe that selectively reacts with peroxynitrite yielding a >200-fold change upon reaction. The probe effectively visualized fluctuations in peroxynitrite generation by arginase 1 in vivo and in vitro. This provides evidence that arginase 1 is a critical regulator of peroxynitrite.

AB - Peroxynitrite, a transient reactive oxygen species (ROS), is believed to play a deleterious role in physiological processes. Herein, we report a two-photon ratiometric fluorescent probe that selectively reacts with peroxynitrite yielding a >200-fold change upon reaction. The probe effectively visualized fluctuations in peroxynitrite generation by arginase 1 in vivo and in vitro. This provides evidence that arginase 1 is a critical regulator of peroxynitrite.

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U2 - 10.1021/acs.analchem.1c00911

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JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 29

ER -

Two-Photon, Ratiometric, Quantitative Fluorescent Probe Reveals Fluctuation of Peroxynitrite Regulated by Arginase 1

Abstract

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