Two members of a widely expressed subfamily of hormone-stimulated adenylyl cyclases

Richard T. Premont, Jianqiang Chen, Hai Wen Ma, Madhavi Ponnapalli, Ravi Iyengar

Research output: Contribution to journalArticlepeer-review

192 Scopus citations

Abstract

cDNA encoding a hormone- and guanine nucleotide-stimulated adenylyl cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] (type 6) from rat liver and kidney has been cloned and expressed. This enzyme is stimulated by forskolin, guanosine 5′-[y-thio]triphosphate, and isoproterenol plus GTP but is not stimulated by βγ subunits of guanine nucleotide-binding proteins. A second form (type 5), which is 75% similar to type 6, has also been cloned. Both types 5 and 6 cDNAs have multiple messages. PCR-based detection of the mRNA for the type 5 and 6 enzymes indicates that both are widely distributed. Homology analyses indicate at least four distinct subfamilies of guanine nucleotide stimulatory protein-regulated adenylyl cyclases. Types 5 and 6 enzymes define one distinct subfamily of mammalian adenylyl cyclases. Diversity of one guanine nucleotide-binding protein-regulated effector may allow different modes of regulation of cell-surface signal transmission.

Original languageEnglish
Pages (from-to)9809-9813
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number20
StatePublished - 15 Oct 1992
Externally publishedYes

Keywords

  • Cloning
  • Expression
  • Guanine nucleotide stimulatory protein-stimulated
  • Kidney
  • Liver

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