Two distinct insulin-related molecules in the guinea pig: immunological and biochemical characterization of insulin-like immunoactivity from extrapancreatic tissues of the guinea pig

J. L. Rosenzweig; D. LeRoith; M. A. Lesniak; C. C. Yip; D. N. Orth; H. R. Nankin; P. Murone; M. Berelowitz; L. A. Frohman; A. S. Liotta; D. T. Krieger; J. Roth

doi:10.1007/BF00282240

Two distinct insulin-related molecules in the guinea pig: immunological and biochemical characterization of insulin-like immunoactivity from extrapancreatic tissues of the guinea pig

J. L. Rosenzweig, D. LeRoith, M. A. Lesniak, C. C. Yip, D. N. Orth, H. R. Nankin, P. Murone, M. Berelowitz, L. A. Frohman, A. S. Liotta, D. T. Krieger, J. Roth

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Abstract

In this study we extracted guinea pig brain and testis with; the extract was adsorbed to and eluted from cartridges (the Sep-Pak C 18 procedure). We found this procedure superior for recovering crystalline insulin added to buffers or tissues, and for recovering endogenous insulin from plasma, but inferior for recovery of insulin from tissues. However, we did find 'rat/pork' type-insulin in guinea pig brain and testis (5-50 pg/g wet weight tissue). Our results with the Sep-Pak C18 procedures were reproduced by four other laboratories (who found 4-60 pg/g wet weight of tissue) and similar findings were also obtained by an independent investigator. Thus, we conclude that extrapancreatic tissues of guinea pigs have a second type of insulin-related material that is more typical of other mammalian insulins, but that the amount recovered is dependent upon the extraction procedure utilized.

Original language	English
Pages (from-to)	237-243
Number of pages	7
Journal	Diabetologia
Volume	28
Issue number	4
DOIs	https://doi.org/10.1007/BF00282240
State	Published - Apr 1985
Externally published	Yes

Keywords

Guinea pig
extrapancreatic tissues
insulin-related immunoactivity

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10.1007/BF00282240

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Rosenzweig, J. L., LeRoith, D., Lesniak, M. A., Yip, C. C., Orth, D. N., Nankin, H. R., Murone, P., Berelowitz, M., Frohman, L. A., Liotta, A. S., Krieger, D. T., & Roth, J. (1985). Two distinct insulin-related molecules in the guinea pig: immunological and biochemical characterization of insulin-like immunoactivity from extrapancreatic tissues of the guinea pig. Diabetologia, 28(4), 237-243. https://doi.org/10.1007/BF00282240

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title = "Two distinct insulin-related molecules in the guinea pig: immunological and biochemical characterization of insulin-like immunoactivity from extrapancreatic tissues of the guinea pig",

abstract = "In this study we extracted guinea pig brain and testis with; the extract was adsorbed to and eluted from cartridges (the Sep-Pak C 18 procedure). We found this procedure superior for recovering crystalline insulin added to buffers or tissues, and for recovering endogenous insulin from plasma, but inferior for recovery of insulin from tissues. However, we did find 'rat/pork' type-insulin in guinea pig brain and testis (5-50 pg/g wet weight tissue). Our results with the Sep-Pak C18 procedures were reproduced by four other laboratories (who found 4-60 pg/g wet weight of tissue) and similar findings were also obtained by an independent investigator. Thus, we conclude that extrapancreatic tissues of guinea pigs have a second type of insulin-related material that is more typical of other mammalian insulins, but that the amount recovered is dependent upon the extraction procedure utilized.",

keywords = "Guinea pig, extrapancreatic tissues, insulin-related immunoactivity",

author = "Rosenzweig, {J. L.} and D. LeRoith and Lesniak, {M. A.} and Yip, {C. C.} and Orth, {D. N.} and Nankin, {H. R.} and P. Murone and M. Berelowitz and Frohman, {L. A.} and Liotta, {A. S.} and Krieger, {D. T.} and J. Roth",

year = "1985",

month = apr,

doi = "10.1007/BF00282240",

language = "English",

volume = "28",

pages = "237--243",

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Rosenzweig, JL, LeRoith, D, Lesniak, MA, Yip, CC, Orth, DN, Nankin, HR, Murone, P, Berelowitz, M, Frohman, LA, Liotta, AS, Krieger, DT & Roth, J 1985, 'Two distinct insulin-related molecules in the guinea pig: immunological and biochemical characterization of insulin-like immunoactivity from extrapancreatic tissues of the guinea pig', Diabetologia, vol. 28, no. 4, pp. 237-243. https://doi.org/10.1007/BF00282240

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T2 - immunological and biochemical characterization of insulin-like immunoactivity from extrapancreatic tissues of the guinea pig

AU - Rosenzweig, J. L.

AU - LeRoith, D.

AU - Lesniak, M. A.

AU - Yip, C. C.

AU - Orth, D. N.

AU - Nankin, H. R.

AU - Murone, P.

AU - Berelowitz, M.

AU - Frohman, L. A.

AU - Liotta, A. S.

AU - Krieger, D. T.

AU - Roth, J.

PY - 1985/4

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AB - In this study we extracted guinea pig brain and testis with; the extract was adsorbed to and eluted from cartridges (the Sep-Pak C 18 procedure). We found this procedure superior for recovering crystalline insulin added to buffers or tissues, and for recovering endogenous insulin from plasma, but inferior for recovery of insulin from tissues. However, we did find 'rat/pork' type-insulin in guinea pig brain and testis (5-50 pg/g wet weight tissue). Our results with the Sep-Pak C18 procedures were reproduced by four other laboratories (who found 4-60 pg/g wet weight of tissue) and similar findings were also obtained by an independent investigator. Thus, we conclude that extrapancreatic tissues of guinea pigs have a second type of insulin-related material that is more typical of other mammalian insulins, but that the amount recovered is dependent upon the extraction procedure utilized.

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Two distinct insulin-related molecules in the guinea pig: immunological and biochemical characterization of insulin-like immunoactivity from extrapancreatic tissues of the guinea pig

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Keywords

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