Two-dimensional electrophoresis and mass spectrometry for protein identification

Amaresh K. Ranjan, Anil Gulati

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations

Abstract

Two-dimensional electrophoresis is useful for separation of individual proteins that can be easily isolated and processed with mass spectrometry for their identification. Here we describe a simplified method of 2D electrophoresis with aim to help beginners in optimization of protein separation protocol, which can be further modified to enhance protein spot resolution. Current protocol can be used in different fields of biology including progenitor cells.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages185-195
Number of pages11
DOIs
StatePublished - 2019
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2029
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • 2D Electrophoresis
  • Isoelectric focusing
  • Mass spectrometry
  • Proteins
  • Proteomics
  • SDS-PAGE

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