@article{bd8d12d2880f41a39360cff3c5be2f35,
title = "Two-color, two-photon uncaging of glutamate and GABA",
abstract = "We developed a caged GABA (γ-aminobutyric acid), which, when combined with an appropriate caged glutamate, allows bimodal control of neuronal membrane potential with subcellular resolution using optically independent two-photon uncaging of each neurotransmitter. We used two-color, two-photon uncaging to fire and block action potentials from rat hippocampal CA1 neurons in brain slices with 720-nm and 830-nm light, respectively. Our method should be generalizable to other chemical messenger pairs.",
author = "Srinivas Kantevari and Masanori Matsuzaki and Yuya Kanemoto and Haruo Kasai and Ellis-Davies, {Graham C.R.}",
note = "Funding Information: This work was supported by the US National Institutes of Health grant GM53395 (to G.C.R.E.-D.) and by Grants-in-Aids for Specially Promoted Area number 2000009 (to H.K.), for Scientific Research on Priority Areas number 20021008 (to M.M.) and for Young Scientist (A) number 19680020 (to M.M.) from the Ministry of Education, Culture, Sports, Science and Technology, Japan, and Precursory Research for Embryonic Science and Technology and Core Research for Evolutional Science and Technology, Japan Science and Technology Agency (to M.M.). In 1993, G.C.R.E.-D. discussed the idea of two-color uncaging with the late Larry Katz, who had just described the first example of glutamate uncaging in brain slices. With advent of nonlinear methods, we have realized that idea.",
year = "2010",
month = feb,
doi = "10.1038/nmeth.1413",
language = "English",
volume = "7",
pages = "123--125",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Research",
number = "2",
}