Two-color, two-photon uncaging of glutamate and GABA

Srinivas Kantevari, Masanori Matsuzaki, Yuya Kanemoto, Haruo Kasai, Graham C.R. Ellis-Davies

Research output: Contribution to journalArticlepeer-review

108 Scopus citations


We developed a caged GABA (γ-aminobutyric acid), which, when combined with an appropriate caged glutamate, allows bimodal control of neuronal membrane potential with subcellular resolution using optically independent two-photon uncaging of each neurotransmitter. We used two-color, two-photon uncaging to fire and block action potentials from rat hippocampal CA1 neurons in brain slices with 720-nm and 830-nm light, respectively. Our method should be generalizable to other chemical messenger pairs.

Original languageEnglish
Pages (from-to)123-125
Number of pages3
JournalNature Methods
Issue number2
StatePublished - Feb 2010
Externally publishedYes


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