Twelve-transmembrane-segment (TMS) version (ΔTMS VII-VIII) of the 14-TMS Tet(L) antibiotic resistance protein retains monovalent cation transport modes but lacks tetracycline efflux capacity

J. Jin, A. A. Guffanti, C. Beck, T. A. Krulwich

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

A "Tet(L)-12" version of Tet(L), a tetracycline efflux protein with 14 transmembrane segments (TMS), was constructed by deletion of two central TMS. Tet(L)-12 catalyzed Na+/H+ antiport and antiport with K+ as a coupling ion as well as or better than wild-type Tet(L) but exhibited no tetracycline-Me2+/H+ antiport in Escherichia coli vesicles.

Original languageEnglish
Pages (from-to)2667-2671
Number of pages5
JournalJournal of Bacteriology
Volume183
Issue number8
DOIs
StatePublished - 2001

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