Tumor suppressor activity of KLF6 mediated by downregulation of the PTTG1 oncogene

Ursula E. Lee; Zahra Ghiassi-Nejad; Andrew J. Paris; Steven Yea; Goutham Narla; Martin Walsh; Scott L. Friedman

doi:10.1016/j.febslet.2010.01.049

Tumor suppressor activity of KLF6 mediated by downregulation of the PTTG1 oncogene

Ursula E. Lee, Zahra Ghiassi-Nejad, Andrew J. Paris, Steven Yea, Goutham Narla, Martin Walsh, Scott L. Friedman

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

The tumor suppressor Kruppel-like factor 6 (KLF6) is frequently inactivated in hepatocellular carcinoma (HCC). To unearth downstream transcriptional targets of KLF6, cDNA microarray analysis of whole liver was compared between KLF6+/+ and KLF6+/- mice. Pituitary tumor transforming gene 1 (PTTG1), an oncogene, was the most up-regulated transcript in KLF6+/- liver. In human HCCs, KLF6 mRNA was significantly decreased, associated with increased PTTG1. In HepG2, KLF6 transcriptionally repressed PTTG1 by direct promoter interaction. Whereas KLF6 downregulation by siRNA increased HepG2 proliferation, siRNA to PTTG1 was anti-proliferative. PTTG1 downregulation represents a novel tumor suppressor pathway of KLF6.

Original language	English
Pages (from-to)	1006-1010
Number of pages	5
Journal	FEBS Letters
Volume	584
Issue number	5
DOIs	https://doi.org/10.1016/j.febslet.2010.01.049
State	Published - 5 Mar 2010

Keywords

Hepatocellular carcinoma
Kruppel-like factor 6
Liver cancer
Pituitary tumor transforming gene 1

Access to Document

10.1016/j.febslet.2010.01.049

Cite this

@article{4cad567194fb4ffc8417969ce11fd5f4,

title = "Tumor suppressor activity of KLF6 mediated by downregulation of the PTTG1 oncogene",

abstract = "The tumor suppressor Kruppel-like factor 6 (KLF6) is frequently inactivated in hepatocellular carcinoma (HCC). To unearth downstream transcriptional targets of KLF6, cDNA microarray analysis of whole liver was compared between KLF6+/+ and KLF6+/- mice. Pituitary tumor transforming gene 1 (PTTG1), an oncogene, was the most up-regulated transcript in KLF6+/- liver. In human HCCs, KLF6 mRNA was significantly decreased, associated with increased PTTG1. In HepG2, KLF6 transcriptionally repressed PTTG1 by direct promoter interaction. Whereas KLF6 downregulation by siRNA increased HepG2 proliferation, siRNA to PTTG1 was anti-proliferative. PTTG1 downregulation represents a novel tumor suppressor pathway of KLF6.",

keywords = "Hepatocellular carcinoma, Kruppel-like factor 6, Liver cancer, Pituitary tumor transforming gene 1",

author = "Lee, {Ursula E.} and Zahra Ghiassi-Nejad and Paris, {Andrew J.} and Steven Yea and Goutham Narla and Martin Walsh and Friedman, {Scott L.}",

year = "2010",

month = mar,

day = "5",

doi = "10.1016/j.febslet.2010.01.049",

language = "English",

volume = "584",

pages = "1006--1010",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "5",

}

TY - JOUR

T1 - Tumor suppressor activity of KLF6 mediated by downregulation of the PTTG1 oncogene

AU - Lee, Ursula E.

AU - Ghiassi-Nejad, Zahra

AU - Paris, Andrew J.

AU - Yea, Steven

AU - Narla, Goutham

AU - Walsh, Martin

AU - Friedman, Scott L.

PY - 2010/3/5

Y1 - 2010/3/5

N2 - The tumor suppressor Kruppel-like factor 6 (KLF6) is frequently inactivated in hepatocellular carcinoma (HCC). To unearth downstream transcriptional targets of KLF6, cDNA microarray analysis of whole liver was compared between KLF6+/+ and KLF6+/- mice. Pituitary tumor transforming gene 1 (PTTG1), an oncogene, was the most up-regulated transcript in KLF6+/- liver. In human HCCs, KLF6 mRNA was significantly decreased, associated with increased PTTG1. In HepG2, KLF6 transcriptionally repressed PTTG1 by direct promoter interaction. Whereas KLF6 downregulation by siRNA increased HepG2 proliferation, siRNA to PTTG1 was anti-proliferative. PTTG1 downregulation represents a novel tumor suppressor pathway of KLF6.

AB - The tumor suppressor Kruppel-like factor 6 (KLF6) is frequently inactivated in hepatocellular carcinoma (HCC). To unearth downstream transcriptional targets of KLF6, cDNA microarray analysis of whole liver was compared between KLF6+/+ and KLF6+/- mice. Pituitary tumor transforming gene 1 (PTTG1), an oncogene, was the most up-regulated transcript in KLF6+/- liver. In human HCCs, KLF6 mRNA was significantly decreased, associated with increased PTTG1. In HepG2, KLF6 transcriptionally repressed PTTG1 by direct promoter interaction. Whereas KLF6 downregulation by siRNA increased HepG2 proliferation, siRNA to PTTG1 was anti-proliferative. PTTG1 downregulation represents a novel tumor suppressor pathway of KLF6.

KW - Hepatocellular carcinoma

KW - Kruppel-like factor 6

KW - Liver cancer

KW - Pituitary tumor transforming gene 1

UR - http://www.scopus.com/inward/record.url?scp=76749161736&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2010.01.049

DO - 10.1016/j.febslet.2010.01.049

M3 - Article

C2 - 20116377

AN - SCOPUS:76749161736

SN - 0014-5793

VL - 584

SP - 1006

EP - 1010

JO - FEBS Letters

JF - FEBS Letters

IS - 5

ER -

Tumor suppressor activity of KLF6 mediated by downregulation of the PTTG1 oncogene

Abstract

Keywords

Access to Document

Fingerprint

Cite this