TY - JOUR
T1 - Tumor necrosis factor-α- and IL-4-independent development of Langerhans cell-like dendritic cells from M-CSF-conditioned precursors
AU - Barbaroux, Jean Baptiste
AU - Kwan, Wing Hong
AU - Allam, Jean Pierre
AU - Novak, Natalija
AU - Bieber, Thomas
AU - Fridman, Wolf H.
AU - Groves, Richard
AU - Mueller, Chris G.
N1 - Funding Information:
We thank Charlotte Boix for technical help, Christophe Combardière for help in calcium-flux analysis, and the Bourg-La-Reine maternity ward for providing umbilical cord blood. J.-B. Barbaroux is the recipient of the Chelsea and Westminster Hospital special trustees grant. This work is supported by INSERM, the Association pour la Recherche contre le Cancer (ARC), and the Skin Treatment and Research Trust.
PY - 2006/1
Y1 - 2006/1
N2 - GM-CSF and transforming growth factor β (TGFβ) are required for the generation of Langerhans cells (LC), members of the dendritic cell (DC) family. Tumor necrosis factor α (TNFα) and IL-4 can enhance LC differentiation from human monocytes or CD34+ progenitors. Here, we show that M-CSF-cultured DC precursors derived from CD34+ progenitors resemble dermal CD14+ cells and readily convert to LC-like DC in GM-CSF/TGFβ. The cells express Langerin, CD1a, and CCR6, migrate in response to CCR6 ligand CCL20, and contain Birbeck granules. TNFα and IL-4, added separately or together, have an inhibitory effect on LC differentiation. Cells differentiated in the presence of IL-4 and TNFα express low levels of CCR7. This suggests that M-CSF-conditioned DC precursors retain the capacity to efficiently undergo a differentiation program, giving rise to LC-like DC solely through the effect of GM-CSF and TGFβ.
AB - GM-CSF and transforming growth factor β (TGFβ) are required for the generation of Langerhans cells (LC), members of the dendritic cell (DC) family. Tumor necrosis factor α (TNFα) and IL-4 can enhance LC differentiation from human monocytes or CD34+ progenitors. Here, we show that M-CSF-cultured DC precursors derived from CD34+ progenitors resemble dermal CD14+ cells and readily convert to LC-like DC in GM-CSF/TGFβ. The cells express Langerin, CD1a, and CCR6, migrate in response to CCR6 ligand CCL20, and contain Birbeck granules. TNFα and IL-4, added separately or together, have an inhibitory effect on LC differentiation. Cells differentiated in the presence of IL-4 and TNFα express low levels of CCR7. This suggests that M-CSF-conditioned DC precursors retain the capacity to efficiently undergo a differentiation program, giving rise to LC-like DC solely through the effect of GM-CSF and TGFβ.
UR - https://www.scopus.com/pages/publications/33644782719
U2 - 10.1038/sj.jid.5700023
DO - 10.1038/sj.jid.5700023
M3 - Article
C2 - 16417226
AN - SCOPUS:33644782719
SN - 0022-202X
VL - 126
SP - 114
EP - 120
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 1
ER -