Tumor localization in nude mice bearing human breast carcinoma xenografts using 111In-DTPA conjugates of monoclonal antibodies

Shrishailam Yemul; Jorge A. Leon; David W. Seldin; Mary Jean Link; Peter Kramer; Ricardo Mesa-Tejada; Alison Estabrook

doi:10.1016/0883-2897(91)90125-5

Tumor localization in nude mice bearing human breast carcinoma xenografts using ¹¹¹In-DTPA conjugates of monoclonal antibodies

Shrishailam Yemul
, Jorge A. Leon
, David W. Seldin
, Mary Jean Link
, Peter Kramer
, Ricardo Mesa-Tejada
, Alison Estabrook

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Biodistribution of monoclonal antibody T43 and its F(ab′)₂ ¹¹¹In-DTPA conjugates were determined in nu/nu mice bearing human breast tumor and rat pituitary tumor xenografts. T43 localized in the target tumor with tumor blood ratios of 3.9 (P < 0.01) and 4.5 (P < 0.05) at 48 and 72 h, respectively. T43 F(ab′)₂ fragments localized with tumor blood ratio of 14.2 (P <0.1) at 72 h. Tumors as small as 4mm were detected without computer subtraction technique. These studies suggest that T43 and T43 F(ab′)₂ might be useful reagents in radioimaging.

Original language	English
Pages (from-to)	295-301,303-304
Journal	Nuclear Medicine and Biology
Volume	18
Issue number	3
DOIs	https://doi.org/10.1016/0883-2897(91)90125-5
State	Published - 1991
Externally published	Yes

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10.1016/0883-2897(91)90125-5

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@article{fd7ee305157b49458f964afda8e14cdf,

title = "Tumor localization in nude mice bearing human breast carcinoma xenografts using 111In-DTPA conjugates of monoclonal antibodies",

abstract = "Biodistribution of monoclonal antibody T43 and its F(ab′)2 111In-DTPA conjugates were determined in nu/nu mice bearing human breast tumor and rat pituitary tumor xenografts. T43 localized in the target tumor with tumor blood ratios of 3.9 (P < 0.01) and 4.5 (P < 0.05) at 48 and 72 h, respectively. T43 F(ab′)2 fragments localized with tumor blood ratio of 14.2 (P <0.1) at 72 h. Tumors as small as 4mm were detected without computer subtraction technique. These studies suggest that T43 and T43 F(ab′)2 might be useful reagents in radioimaging.",

author = "Shrishailam Yemul and Leon, \{Jorge A.\} and Seldin, \{David W.\} and Link, \{Mary Jean\} and Peter Kramer and Ricardo Mesa-Tejada and Alison Estabrook",

note = "Funding Information: Acknowledgements-We thankD C Tom Parish for supplying the antibodiesT 43 and UR04, Irene Ho and Eileen Kelley for technicaal ssistanceT.h is researchw as supported in part by Johnson \& Johnson SpecialB iologicalP rojects, Irving A. Hansen Memorial Foundation, Florence and Herbert Irving Foundation and NIH Grant 5ROl CA 32984-07.",

year = "1991",

doi = "10.1016/0883-2897(91)90125-5",

language = "English",

volume = "18",

pages = "295--301,303--304",

journal = "Nuclear Medicine and Biology",

issn = "0883-2897",

publisher = "Elsevier Inc.",

number = "3",

}

TY - JOUR

T1 - Tumor localization in nude mice bearing human breast carcinoma xenografts using 111In-DTPA conjugates of monoclonal antibodies

AU - Yemul, Shrishailam

AU - Leon, Jorge A.

AU - Seldin, David W.

AU - Link, Mary Jean

AU - Kramer, Peter

AU - Mesa-Tejada, Ricardo

AU - Estabrook, Alison

N1 - Funding Information: Acknowledgements-We thankD C Tom Parish for supplying the antibodiesT 43 and UR04, Irene Ho and Eileen Kelley for technicaal ssistanceT.h is researchw as supported in part by Johnson & Johnson SpecialB iologicalP rojects, Irving A. Hansen Memorial Foundation, Florence and Herbert Irving Foundation and NIH Grant 5ROl CA 32984-07.

PY - 1991

Y1 - 1991

N2 - Biodistribution of monoclonal antibody T43 and its F(ab′)2 111In-DTPA conjugates were determined in nu/nu mice bearing human breast tumor and rat pituitary tumor xenografts. T43 localized in the target tumor with tumor blood ratios of 3.9 (P < 0.01) and 4.5 (P < 0.05) at 48 and 72 h, respectively. T43 F(ab′)2 fragments localized with tumor blood ratio of 14.2 (P <0.1) at 72 h. Tumors as small as 4mm were detected without computer subtraction technique. These studies suggest that T43 and T43 F(ab′)2 might be useful reagents in radioimaging.

AB - Biodistribution of monoclonal antibody T43 and its F(ab′)2 111In-DTPA conjugates were determined in nu/nu mice bearing human breast tumor and rat pituitary tumor xenografts. T43 localized in the target tumor with tumor blood ratios of 3.9 (P < 0.01) and 4.5 (P < 0.05) at 48 and 72 h, respectively. T43 F(ab′)2 fragments localized with tumor blood ratio of 14.2 (P <0.1) at 72 h. Tumors as small as 4mm were detected without computer subtraction technique. These studies suggest that T43 and T43 F(ab′)2 might be useful reagents in radioimaging.

UR - https://www.scopus.com/pages/publications/44949284647

U2 - 10.1016/0883-2897(91)90125-5

DO - 10.1016/0883-2897(91)90125-5

M3 - Article

C2 - 2071442

AN - SCOPUS:44949284647

SN - 0883-2897

VL - 18

SP - 295-301,303-304

JO - Nuclear Medicine and Biology

JF - Nuclear Medicine and Biology

IS - 3

ER -

Tumor localization in nude mice bearing human breast carcinoma xenografts using 111In-DTPA conjugates of monoclonal antibodies

Abstract

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Tumor localization in nude mice bearing human breast carcinoma xenografts using ¹¹¹In-DTPA conjugates of monoclonal antibodies