Abstract
We have optimized the conditions for efficient NIH3T3 focus formation by calcium phosphate transfection of proviral Abelson-murine leukemia virus (A-MuLV) plasmid DNA. Linearized pA-MuLV, P120 or P160 strains, when transfected with calf thymus carrier DNA, will produce 40-50 foci/100 ng pA-MuLV without cotransfecting Moloney-murine leukemia virus (MoMuLV) plasmid DNA.
| Original language | English |
|---|---|
| Pages (from-to) | 1411-1413 |
| Number of pages | 3 |
| Journal | Oncogene |
| Volume | 4 |
| Issue number | 11 |
| State | Published - 1989 |
| Externally published | Yes |