Transfer of genetic information via isolated amphibian metaphase chromosomes

Barry S. Rosenstein; Betsy M. Ohlsson-Wilhelm

doi:10.1007/BF01542847

Transfer of genetic information via isolated amphibian metaphase chromosomes

Barry S. Rosenstein, Betsy M. Ohlsson-Wilhelm

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Abstract

The metaphase chromosome transfer system of McBride and Ozer (1973) has been adapted to a haploid, euploid, frog cell line. Genes coding for a deoxypyrimidine kinase and an enzyme responsible for a thymidine-specific saturable transport system have each been transferred at frequencies between 10^-6 and 10^-5 transferents per cell treated. Revenants for each of these two genes were observed at frequencies between 10^-8 and 10^-7 revenants per cell tested. Selfing controls showed no transferents. Two colonies were obtained in which cotransfer of both genes may have occurred. Activities of the transferred genes were assayed by incorporation of [³H]thymidine into alkali-stable, acid-precipitable material. Growth properties of 13 transferents in various media were also determined and presence of the appropriate enzymes inferred. These transferents were tested for stability early (25 generations) after transfer and were found to be stable. All 13 transferents possess the normal haploid number of chromosomes (n=13) with no cytologically detectable chromosomal fragments.

Original language	English
Pages (from-to)	341-353
Number of pages	13
Journal	Somatic Cell Genetics
Volume	4
Issue number	3
DOIs	https://doi.org/10.1007/BF01542847
State	Published - May 1978
Externally published	Yes

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title = "Transfer of genetic information via isolated amphibian metaphase chromosomes",

abstract = "The metaphase chromosome transfer system of McBride and Ozer (1973) has been adapted to a haploid, euploid, frog cell line. Genes coding for a deoxypyrimidine kinase and an enzyme responsible for a thymidine-specific saturable transport system have each been transferred at frequencies between 10-6 and 10-5 transferents per cell treated. Revenants for each of these two genes were observed at frequencies between 10-8 and 10-7 revenants per cell tested. Selfing controls showed no transferents. Two colonies were obtained in which cotransfer of both genes may have occurred. Activities of the transferred genes were assayed by incorporation of [3H]thymidine into alkali-stable, acid-precipitable material. Growth properties of 13 transferents in various media were also determined and presence of the appropriate enzymes inferred. These transferents were tested for stability early (25 generations) after transfer and were found to be stable. All 13 transferents possess the normal haploid number of chromosomes (n=13) with no cytologically detectable chromosomal fragments.",

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year = "1978",

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T1 - Transfer of genetic information via isolated amphibian metaphase chromosomes

AU - Rosenstein, Barry S.

AU - Ohlsson-Wilhelm, Betsy M.

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N2 - The metaphase chromosome transfer system of McBride and Ozer (1973) has been adapted to a haploid, euploid, frog cell line. Genes coding for a deoxypyrimidine kinase and an enzyme responsible for a thymidine-specific saturable transport system have each been transferred at frequencies between 10-6 and 10-5 transferents per cell treated. Revenants for each of these two genes were observed at frequencies between 10-8 and 10-7 revenants per cell tested. Selfing controls showed no transferents. Two colonies were obtained in which cotransfer of both genes may have occurred. Activities of the transferred genes were assayed by incorporation of [3H]thymidine into alkali-stable, acid-precipitable material. Growth properties of 13 transferents in various media were also determined and presence of the appropriate enzymes inferred. These transferents were tested for stability early (25 generations) after transfer and were found to be stable. All 13 transferents possess the normal haploid number of chromosomes (n=13) with no cytologically detectable chromosomal fragments.

AB - The metaphase chromosome transfer system of McBride and Ozer (1973) has been adapted to a haploid, euploid, frog cell line. Genes coding for a deoxypyrimidine kinase and an enzyme responsible for a thymidine-specific saturable transport system have each been transferred at frequencies between 10-6 and 10-5 transferents per cell treated. Revenants for each of these two genes were observed at frequencies between 10-8 and 10-7 revenants per cell tested. Selfing controls showed no transferents. Two colonies were obtained in which cotransfer of both genes may have occurred. Activities of the transferred genes were assayed by incorporation of [3H]thymidine into alkali-stable, acid-precipitable material. Growth properties of 13 transferents in various media were also determined and presence of the appropriate enzymes inferred. These transferents were tested for stability early (25 generations) after transfer and were found to be stable. All 13 transferents possess the normal haploid number of chromosomes (n=13) with no cytologically detectable chromosomal fragments.

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Transfer of genetic information via isolated amphibian metaphase chromosomes

Abstract

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