TY - JOUR
T1 - Transfer of Epstein-Barr virus receptors to receptor-negative cells permits virus penetration and antigen expression
AU - Volsky, D. J.
AU - Shapiro, I. M.
AU - Klein, G.
PY - 1980
Y1 - 1980
N2 - Epstein-Barr virus (EBV) receptors were implanted into the membranes of receptor-negative cells, using Sendai virus envelopes as vehicles. The presence of the receptors in the target cell membrane was demonstrated by monitoring the fate of radioiodinated donor membranes. Receptors could be detected for at least 36 hr after implantation. [3H]Thymidine-labeled EBV bound efficiently to receptor-implanted cells but not to control cells. Binding was inhibited by an excess of nonlabeled virus. Of the [3H]thymidine-labeled EBV DNA, 50-75% was found inside the receptor-implanted, EBV-exposed cells 24 hr after the infection. The viral genome was functionally active in B lymphocyte-derived cell lines of human, murine, and baboon origin; in T lymphocyte-derived lines of human and murine origin; in mouse fibroblasts; and in freshly explanted mouse lymphocytes, as shown by the expression of EBV-determined nuclear, early, and viral capsid antigens.
AB - Epstein-Barr virus (EBV) receptors were implanted into the membranes of receptor-negative cells, using Sendai virus envelopes as vehicles. The presence of the receptors in the target cell membrane was demonstrated by monitoring the fate of radioiodinated donor membranes. Receptors could be detected for at least 36 hr after implantation. [3H]Thymidine-labeled EBV bound efficiently to receptor-implanted cells but not to control cells. Binding was inhibited by an excess of nonlabeled virus. Of the [3H]thymidine-labeled EBV DNA, 50-75% was found inside the receptor-implanted, EBV-exposed cells 24 hr after the infection. The viral genome was functionally active in B lymphocyte-derived cell lines of human, murine, and baboon origin; in T lymphocyte-derived lines of human and murine origin; in mouse fibroblasts; and in freshly explanted mouse lymphocytes, as shown by the expression of EBV-determined nuclear, early, and viral capsid antigens.
UR - http://www.scopus.com/inward/record.url?scp=0019207807&partnerID=8YFLogxK
U2 - 10.1073/pnas.77.9.5453
DO - 10.1073/pnas.77.9.5453
M3 - Article
C2 - 6254082
AN - SCOPUS:0019207807
SN - 0027-8424
VL - 77
SP - 5453
EP - 5457
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 9 II
ER -