Transcriptome Analysis of Energy Supply Process During Seed Germination in Phyllostachys edulis

Juan Li, Xiangyu Li, Yucong Bai, Yali Xie, Long Li, Shaohua Mu, Jian Gao

Research output: Contribution to journalArticlepeer-review

Abstract

Energy production plays an important role in seed germination. Elucidating the molecular mechanisms in the main pathways and the energy supply processes that control the germination of Phyllostachys edulis seeds could provide necessary information for the genomic-level improvement of seed crops. In this study, we performed ultrastructural observations and measured the respiration rate, total starch content, crude oil content, and enzymes related to respiratory activities. Transcriptome sequences at five different stages of germination were obtained and analyzed. First, carbohydrate degradation, including lipid and starch metabolism, plays an important role in providing energy during P. edulis seed germination. The total starch and crude oil contents decreased, and starch metabolism and lipid body degradation pathway genes were consistently upregulated during P. edulis seed germination. Second, the glycolytic pathway and citrate cycle in the respiration pathway supply energy during P. edulis seed germination. The respiration intensity increased significantly, with the 6-phosphofructokinase (FPK) enzyme peaking in stage II. Promitochondria broke down into smaller mitochondria, and respiration intensity increased in stages III to IV, with glucose-6-phosphate dehydrogenase and NAD-malate dehydrogenase enzymes reaching peak values in these stages. The AP2 and bHLH proteins were the most commonly upregulated transcription factors related to seed germination and may play significant roles in seed germination.

Original languageEnglish
Pages (from-to)489-511
Number of pages23
JournalPlant Molecular Biology Reporter
Volume41
Issue number3
DOIs
StatePublished - Sep 2023
Externally publishedYes

Keywords

  • Carbohydrate degradation
  • Energy supply
  • Phyllostachys edulis
  • Respiration pathways
  • Transcription factor

Fingerprint

Dive into the research topics of 'Transcriptome Analysis of Energy Supply Process During Seed Germination in Phyllostachys edulis'. Together they form a unique fingerprint.

Cite this