Transcriptional Stability of Cultured Cells upon Prion Infection

Christian Julius, Gregor Hutter, Ulrich Wagner, Harald Seeger, Veronika Kana, Jan Kranich, Peter Klöhn, Charles Weissmann, Gino Miele, Adriano Aguzzi

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Prion infections induce severe disruption of the central nervous system with neuronal vacuolation and extensive glial reactions, and invariably lead to death of affected individuals. The molecular underpinnings of these events are not well understood. To better define the molecular consequences of prion infections, we analyzed the transcriptional response to persistent prion infection in a panel of three murine neural cell lines in vitro. Colony spot immunochemistry assays indicated that 65-100% of cells were infected in each line. Only the Nav1 gene was marginally modulated in one cell line, whereas transcripts previously reported to be derailed in prion-infected cells were not confirmed in the present study. We attribute these discrepancies to the experimental stringency of the current study, which was performed under conditions designed to minimize potential genetic drifts. These findings are at striking variance with gene expression studies performed on whole brains upon prion infections in vivo, suggesting that many of the latter changes represent secondary reactions to infection. We conclude that, suprisingly, there are no universal transcriptional changes induced by prion infection of neural cells in vitro.

Original languageEnglish
Pages (from-to)1222-1233
Number of pages12
JournalJournal of Molecular Biology
Volume375
Issue number5
DOIs
StatePublished - 1 Feb 2008
Externally publishedYes

Keywords

  • PrP
  • RNA microarray
  • gene expression profiling
  • prion infection
  • quantitative real-time PCR

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