Transcription of nucleosomes from human chromatin

Phyllis A. Shaw; Chintaman G. Sahasrabuddhe; Henry G. Hodo; Grady F. Saunders

doi:10.1093/nar/5.8.2999

Transcription of nucleosomes from human chromatin

Phyllis A. Shaw
, Chintaman G. Sahasrabuddhe
, Henry G. Hodo
, Grady F. Saunders

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Nucleosomes (chromatin subunits) prepared by micrococcal nuclease digestion of human nuclei are similar in histone content but substantially reduced in non-histone proteins as compared to undigested chromatin. Chromatin transcription experiments indicate that the DNA in the nucleosomes is accessible to DNA-dependent RNA polymerase in vitro The template capacities of chromatin and nucleosomes are 1.5 and 10%, respectively, relative to high molecular weight DNA, with intermediate values for oligonucleosomes. Three distinct sizes of transcripts, 150, 120 and 95 nucleotides in length, are obtained when nucleosomes are used as templates. However, when nucleosom DNA is used as a template, the predominant size of transcripts is 150 nucleotides. When oligonucleosomes are used as templates longer transcripts are obtained. This indicates that RNA polymerase can transcribe the DNA con tained in the nucleosomes.

Original language	English
Pages (from-to)	2999-3012
Number of pages	14
Journal	Nucleic Acids Research
Volume	5
Issue number	8
DOIs	https://doi.org/10.1093/nar/5.8.2999
State	Published - Aug 1978
Externally published	Yes

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title = "Transcription of nucleosomes from human chromatin",

abstract = "Nucleosomes (chromatin subunits) prepared by micrococcal nuclease digestion of human nuclei are similar in histone content but substantially reduced in non-histone proteins as compared to undigested chromatin. Chromatin transcription experiments indicate that the DNA in the nucleosomes is accessible to DNA-dependent RNA polymerase in vitro The template capacities of chromatin and nucleosomes are 1.5 and 10\%, respectively, relative to high molecular weight DNA, with intermediate values for oligonucleosomes. Three distinct sizes of transcripts, 150, 120 and 95 nucleotides in length, are obtained when nucleosomes are used as templates. However, when nucleosom DNA is used as a template, the predominant size of transcripts is 150 nucleotides. When oligonucleosomes are used as templates longer transcripts are obtained. This indicates that RNA polymerase can transcribe the DNA con tained in the nucleosomes.",

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T1 - Transcription of nucleosomes from human chromatin

AU - Shaw, Phyllis A.

AU - Sahasrabuddhe, Chintaman G.

AU - Hodo, Henry G.

AU - Saunders, Grady F.

PY - 1978/8

Y1 - 1978/8

N2 - Nucleosomes (chromatin subunits) prepared by micrococcal nuclease digestion of human nuclei are similar in histone content but substantially reduced in non-histone proteins as compared to undigested chromatin. Chromatin transcription experiments indicate that the DNA in the nucleosomes is accessible to DNA-dependent RNA polymerase in vitro The template capacities of chromatin and nucleosomes are 1.5 and 10%, respectively, relative to high molecular weight DNA, with intermediate values for oligonucleosomes. Three distinct sizes of transcripts, 150, 120 and 95 nucleotides in length, are obtained when nucleosomes are used as templates. However, when nucleosom DNA is used as a template, the predominant size of transcripts is 150 nucleotides. When oligonucleosomes are used as templates longer transcripts are obtained. This indicates that RNA polymerase can transcribe the DNA con tained in the nucleosomes.

AB - Nucleosomes (chromatin subunits) prepared by micrococcal nuclease digestion of human nuclei are similar in histone content but substantially reduced in non-histone proteins as compared to undigested chromatin. Chromatin transcription experiments indicate that the DNA in the nucleosomes is accessible to DNA-dependent RNA polymerase in vitro The template capacities of chromatin and nucleosomes are 1.5 and 10%, respectively, relative to high molecular weight DNA, with intermediate values for oligonucleosomes. Three distinct sizes of transcripts, 150, 120 and 95 nucleotides in length, are obtained when nucleosomes are used as templates. However, when nucleosom DNA is used as a template, the predominant size of transcripts is 150 nucleotides. When oligonucleosomes are used as templates longer transcripts are obtained. This indicates that RNA polymerase can transcribe the DNA con tained in the nucleosomes.

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DO - 10.1093/nar/5.8.2999

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JO - Nucleic Acids Research

JF - Nucleic Acids Research

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Transcription of nucleosomes from human chromatin

Abstract

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