Transcription Factor Levels after Forward Programming of Human Pluripotent Stem Cells with GATA1, FLI1, and TAL1 Determine Megakaryocyte versus Erythroid Cell Fate Decision

Amanda Dalby; Jose Ballester-Beltrán; Chiara Lincetto; Annett Mueller; Nicola Foad; Amanda Evans; James Baye; Ernest Turro; Thomas Moreau; Marloes R. Tijssen; Cedric Ghevaert

doi:10.1016/j.stemcr.2018.11.001

Transcription Factor Levels after Forward Programming of Human Pluripotent Stem Cells with GATA1, FLI1, and TAL1 Determine Megakaryocyte versus Erythroid Cell Fate Decision

Amanda Dalby, Jose Ballester-Beltrán, Chiara Lincetto, Annett Mueller, Nicola Foad, Amanda Evans, James Baye, Ernest Turro, Thomas Moreau, Marloes R. Tijssen, Cedric Ghevaert

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The authors show that overexpression of GATA1, TAL1, and FLI1 in hPSCs leads to the formation of progenitors that are committed early during differentiation to either the megakaryocytic or erythroid lineage. The cell fate decision is independent of the cytokines used (TPO or EPO) but is dictated by the level of the FLI1 transgene.

Original language	English
Pages (from-to)	1462-1478
Number of pages	17
Journal	Stem Cell Reports
Volume	11
Issue number	6
DOIs	https://doi.org/10.1016/j.stemcr.2018.11.001
State	Published - 11 Dec 2018
Externally published	Yes

Keywords

erythroblast
forward programming
lineage fate decision
megakaryocyte
pluripotent stem cells

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10.1016/j.stemcr.2018.11.001

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Dalby, A., Ballester-Beltrán, J., Lincetto, C., Mueller, A., Foad, N., Evans, A., Baye, J., Turro, E., Moreau, T., Tijssen, M. R., & Ghevaert, C. (2018). Transcription Factor Levels after Forward Programming of Human Pluripotent Stem Cells with GATA1, FLI1, and TAL1 Determine Megakaryocyte versus Erythroid Cell Fate Decision. Stem Cell Reports, 11(6), 1462-1478. https://doi.org/10.1016/j.stemcr.2018.11.001

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title = "Transcription Factor Levels after Forward Programming of Human Pluripotent Stem Cells with GATA1, FLI1, and TAL1 Determine Megakaryocyte versus Erythroid Cell Fate Decision",

abstract = "The authors show that overexpression of GATA1, TAL1, and FLI1 in hPSCs leads to the formation of progenitors that are committed early during differentiation to either the megakaryocytic or erythroid lineage. The cell fate decision is independent of the cytokines used (TPO or EPO) but is dictated by the level of the FLI1 transgene.",

keywords = "erythroblast, forward programming, lineage fate decision, megakaryocyte, pluripotent stem cells",

author = "Amanda Dalby and Jose Ballester-Beltr{\'a}n and Chiara Lincetto and Annett Mueller and Nicola Foad and Amanda Evans and James Baye and Ernest Turro and Thomas Moreau and Tijssen, {Marloes R.} and Cedric Ghevaert",

note = "Publisher Copyright: {\textcopyright} 2018",

year = "2018",

month = dec,

day = "11",

doi = "10.1016/j.stemcr.2018.11.001",

language = "English",

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Dalby, A, Ballester-Beltrán, J, Lincetto, C, Mueller, A, Foad, N, Evans, A, Baye, J, Turro, E, Moreau, T, Tijssen, MR & Ghevaert, C 2018, 'Transcription Factor Levels after Forward Programming of Human Pluripotent Stem Cells with GATA1, FLI1, and TAL1 Determine Megakaryocyte versus Erythroid Cell Fate Decision', Stem Cell Reports, vol. 11, no. 6, pp. 1462-1478. https://doi.org/10.1016/j.stemcr.2018.11.001

Transcription Factor Levels after Forward Programming of Human Pluripotent Stem Cells with GATA1, FLI1, and TAL1 Determine Megakaryocyte versus Erythroid Cell Fate Decision. / Dalby, Amanda; Ballester-Beltrán, Jose; Lincetto, Chiara et al.
In: Stem Cell Reports, Vol. 11, No. 6, 11.12.2018, p. 1462-1478.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Transcription Factor Levels after Forward Programming of Human Pluripotent Stem Cells with GATA1, FLI1, and TAL1 Determine Megakaryocyte versus Erythroid Cell Fate Decision

AU - Dalby, Amanda

AU - Ballester-Beltrán, Jose

AU - Lincetto, Chiara

AU - Mueller, Annett

AU - Foad, Nicola

AU - Evans, Amanda

AU - Baye, James

AU - Turro, Ernest

AU - Moreau, Thomas

AU - Tijssen, Marloes R.

AU - Ghevaert, Cedric

PY - 2018/12/11

Y1 - 2018/12/11

N2 - The authors show that overexpression of GATA1, TAL1, and FLI1 in hPSCs leads to the formation of progenitors that are committed early during differentiation to either the megakaryocytic or erythroid lineage. The cell fate decision is independent of the cytokines used (TPO or EPO) but is dictated by the level of the FLI1 transgene.

AB - The authors show that overexpression of GATA1, TAL1, and FLI1 in hPSCs leads to the formation of progenitors that are committed early during differentiation to either the megakaryocytic or erythroid lineage. The cell fate decision is independent of the cytokines used (TPO or EPO) but is dictated by the level of the FLI1 transgene.

KW - erythroblast

KW - forward programming

KW - lineage fate decision

KW - megakaryocyte

KW - pluripotent stem cells

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U2 - 10.1016/j.stemcr.2018.11.001

DO - 10.1016/j.stemcr.2018.11.001

M3 - Article

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AN - SCOPUS:85058612559

SN - 2213-6711

VL - 11

SP - 1462

EP - 1478

JO - Stem Cell Reports

JF - Stem Cell Reports

IS - 6

ER -

Transcription Factor Levels after Forward Programming of Human Pluripotent Stem Cells with GATA1, FLI1, and TAL1 Determine Megakaryocyte versus Erythroid Cell Fate Decision

Abstract

Keywords

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