Tracking N-acetyllactosamine on cell-surface glycans in vivo

Tianqing Zheng; Hao Jiang; Marilyn Gros; David Soriano Del Amo; Subha Sundaram; Grégoire Lauvau; Florence Marlow; Yi Liu; Pamela Stanley; Peng Wu

doi:10.1002/anie.201100265

Tracking N-acetyllactosamine on cell-surface glycans in vivo

Tianqing Zheng
, Hao Jiang
, Marilyn Gros
, David Soriano Del Amo
, Subha Sundaram
, Grégoire Lauvau
, Florence Marlow
, Yi Liu
, Pamela Stanley
, Peng Wu

Research output: Contribution to journal › Article › peer-review

79 Scopus citations

Abstract

In living color: Many mammalian glycans associated with signaling receptors contain terminal or penultimate N-acetyllactosamine. A highly specific method for labeling this disaccharide on cell-surface glycoproteins of live cultured cells and zebrafish embryos is reported. The two-step chemoenzymatic approach involves in situ fucosylation followed by a bioorthogonal click reaction (see scheme; α(1,3)FucT=α(1,3)-fucosyltransferase).

Original language	English
Pages (from-to)	4113-4118
Number of pages	6
Journal	Angewandte Chemie - International Edition
Volume	50
Issue number	18
DOIs	https://doi.org/10.1002/anie.201100265
State	Published - 26 Apr 2011
Externally published	Yes

Keywords

click chemistry
enzyme catalysis
glycoconjugates
glycosylation
transferases

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10.1002/anie.201100265

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title = "Tracking N-acetyllactosamine on cell-surface glycans in vivo",

abstract = "In living color: Many mammalian glycans associated with signaling receptors contain terminal or penultimate N-acetyllactosamine. A highly specific method for labeling this disaccharide on cell-surface glycoproteins of live cultured cells and zebrafish embryos is reported. The two-step chemoenzymatic approach involves in situ fucosylation followed by a bioorthogonal click reaction (see scheme; α(1,3)FucT=α(1,3)-fucosyltransferase).",

keywords = "click chemistry, enzyme catalysis, glycoconjugates, glycosylation, transferases",

author = "Tianqing Zheng and Hao Jiang and Marilyn Gros and \{Soriano Del Amo\}, David and Subha Sundaram and Gr{\'e}goire Lauvau and Florence Marlow and Yi Liu and Pamela Stanley and Peng Wu",

year = "2011",

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doi = "10.1002/anie.201100265",

language = "English",

volume = "50",

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T1 - Tracking N-acetyllactosamine on cell-surface glycans in vivo

AU - Zheng, Tianqing

AU - Jiang, Hao

AU - Gros, Marilyn

AU - Soriano Del Amo, David

AU - Sundaram, Subha

AU - Lauvau, Grégoire

AU - Marlow, Florence

AU - Liu, Yi

AU - Stanley, Pamela

AU - Wu, Peng

PY - 2011/4/26

Y1 - 2011/4/26

N2 - In living color: Many mammalian glycans associated with signaling receptors contain terminal or penultimate N-acetyllactosamine. A highly specific method for labeling this disaccharide on cell-surface glycoproteins of live cultured cells and zebrafish embryos is reported. The two-step chemoenzymatic approach involves in situ fucosylation followed by a bioorthogonal click reaction (see scheme; α(1,3)FucT=α(1,3)-fucosyltransferase).

AB - In living color: Many mammalian glycans associated with signaling receptors contain terminal or penultimate N-acetyllactosamine. A highly specific method for labeling this disaccharide on cell-surface glycoproteins of live cultured cells and zebrafish embryos is reported. The two-step chemoenzymatic approach involves in situ fucosylation followed by a bioorthogonal click reaction (see scheme; α(1,3)FucT=α(1,3)-fucosyltransferase).

KW - click chemistry

KW - enzyme catalysis

KW - glycoconjugates

KW - glycosylation

KW - transferases

UR - https://www.scopus.com/pages/publications/79955390922

U2 - 10.1002/anie.201100265

DO - 10.1002/anie.201100265

M3 - Article

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SN - 1433-7851

VL - 50

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EP - 4118

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 18

ER -

Tracking N-acetyllactosamine on cell-surface glycans in vivo

Abstract

Keywords

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