Tough Decoy-Mediated Cardiac Gene Suppression

Changwon Kho

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

MicroRNA (miRNA) is a small, non-coding RNA molecule (~22 nucleotides) that acts as a post-transcriptional gene regulator, primarily by inhibiting the translation of target mRNA transcripts or affecting cell mRNA stability. Since miRNAs are comprehensively involved in gene regulation, their abnormalities are associated with various human diseases, including cardiovascular disease. Additionally, targeted inhibition of disease-related miRNAs and their targets should have therapeutic potential. Therefore, this chapter describes the experimental steps for targeted inhibition of specific miRNAs using adenoviral vectorized tough decoys that efficiently silence miRNA function in cardiac cells.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages13-30
Number of pages18
DOIs
StatePublished - 2022
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2573
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Adenoviral vectors
  • Functional assays of miRNA inhibition
  • MicroRNA
  • Tough decoy inhibitors

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