Tissue-specific features of the T cell repertoire after allogeneic hematopoietic cell transplantation in human and mouse

Susan DeWolf, Yuval Elhanati, Katherine Nichols, Nicholas R. Waters, Chi L. Nguyen, John B. Slingerland, Natasia Rodriguez, Olga Lyudovyk, Paul A. Giardina, Anastasia I. Kousa, Hana Andrlová, Nick Ceglia, Teng Fei, Rajya Kappagantula, Yanyun Li, Nathan Aleynick, Priscilla Baez, Rajmohan Murali, Akimasa Hayashi, Nicole LeeBrianna Gipson, Madhumitha Rangesa, Zoe Katsamakis, Anqi Dai, Amanda G. Blouin, Maria Arcila, Ignas Masilionis, Ronan Chaligne, Doris M. Ponce, Heather J. Landau, Ioannis Politikos, Roni Tamari, Alan M. Hanash, Robert R. Jenq, Sergio A. Giralt, Kate A. Markey, Yanming Zhang, Miguel Angel Perales, Nicholas D. Socci, Benjamin D. Greenbaum, Christine A. Iacobuzio-Donahue, Travis J. Hollmann, Marcel R.M. van den Brink, Jonathan U. Peled

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

T cells are the central drivers of many inflammatory diseases, but the repertoire of tissue-resident T cells at sites of pathology in human organs remains poorly understood. We examined the site-specificity of T cell receptor (TCR) repertoires across tissues (5 to 18 tissues per patient) in prospectively collected autopsies of patients with and without graft-versus-host disease (GVHD), a potentially lethal tissue-targeting complication of allogeneic hematopoietic cell transplantation, and in mouse models of GVHD. Anatomic similarity between tissues was a key determinant of TCR repertoire composition within patients, independent of disease or transplant status. The T cells recovered from peripheral blood and spleens in patients and mice captured a limited portion of the TCR repertoire detected in tissues. Whereas few T cell clones were shared across patients, motif-based clustering revealed shared repertoire signatures across patients in a tissue-specific fashion. T cells at disease sites had a tissue-resident phenotype and were of donor origin based on single-cell chimerism analysis. These data demonstrate the complex composition of T cell populations that persist in human tissues at the end stage of an inflammatory disorder after lymphocyte-directed therapy. These findings also underscore the importance of studying T cell in tissues rather than blood for tissue-based pathologies and suggest the tissue-specific nature of both the endogenous and posttransplant T cell landscape.

Original languageEnglish
Article numbereabq0476
JournalScience Translational Medicine
Volume15
Issue number706
DOIs
StatePublished - 26 Jul 2023
Externally publishedYes

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