Three-dimensional cell-based microarrays: printing pluripotent stem cells into 3D microenvironments

Jorge F. Pascoal, Tiago G. Fernandes, Gregory J. Nierode, Maria Margarida Diogo, Jonathan S. Dordick, Joaquim M.S. Cabral

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

4 Scopus citations

Abstract

Cell-based microarrays are valuable platforms for the study of cytotoxicity and cellular microenvironment because they enable high-throughput screening of large sets of conditions at reduced reagent consumption. However, most of the described microarray technologies have been applied to two-dimensional cultures, which do not accurately emulate the in vivo three-dimensional (3D) cell–cell and cell–extracellular matrix interactions. Herein, we describe the methodology for production of alginate- and Matrigel-based 3-D cell microarrays for the study of mouse and human pluripotent stem cells on two different chip-based platforms. We further provide protocols for on-chip proliferation/viability analysis and the assessment of protein expression by immunofluorescence.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages69-81
Number of pages13
DOIs
StatePublished - 2018
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1771
ISSN (Print)1064-3745

Keywords

  • 3D cell microarray
  • Cellular microenvironment
  • Cytotoxicity
  • High-content screening
  • Pluripotent stem cells

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