@inbook{ed339d56180944f2862afc287650f0db,
title = "Three-dimensional cell-based microarrays: printing pluripotent stem cells into 3D microenvironments",
abstract = "Cell-based microarrays are valuable platforms for the study of cytotoxicity and cellular microenvironment because they enable high-throughput screening of large sets of conditions at reduced reagent consumption. However, most of the described microarray technologies have been applied to two-dimensional cultures, which do not accurately emulate the in vivo three-dimensional (3D) cell–cell and cell–extracellular matrix interactions. Herein, we describe the methodology for production of alginate- and Matrigel-based 3-D cell microarrays for the study of mouse and human pluripotent stem cells on two different chip-based platforms. We further provide protocols for on-chip proliferation/viability analysis and the assessment of protein expression by immunofluorescence.",
keywords = "3D cell microarray, Cellular microenvironment, Cytotoxicity, High-content screening, Pluripotent stem cells",
author = "Pascoal, {Jorge F.} and Fernandes, {Tiago G.} and Nierode, {Gregory J.} and Diogo, {Maria Margarida} and Dordick, {Jonathan S.} and Cabral, {Joaquim M.S.}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2018.",
year = "2018",
doi = "10.1007/978-1-4939-7792-5_6",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "69--81",
booktitle = "Methods in Molecular Biology",
}