The yvaJ gene of Bacillus subtills encodes a 3'-to-5' exoribonuclease and is not essential in a strain lacking polynucleotide phosphorylase

I. A. Oussenko, D. H. Bechhofer

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Studies of Bacillus subtilis RNases that are involved in mRNA degradation reveal a different pattern from that of Escherichia coli. A strain lacking polynucleotide phosphorylase, the major 3'-to-5' exoribonuclease activity in cell extracts, is viable. Here, we show that the B. subtilis yvaJ gene encodes a second 3'-to-5' exoribonuclease. A strain lacking both of these RNases grows slowly but is viable. The existence of another, as yet unknown, 3'-to-5' exoribonuclease in B. subtilis is suggested.

Original languageEnglish
Pages (from-to)2639-2642
Number of pages4
JournalJournal of Bacteriology
Volume182
Issue number9
DOIs
StatePublished - May 2000
Externally publishedYes

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