THE UPTAKE, METABOLISM AND RELEASE OF HOMOCHOLINE: STUDIES WITH RAT BRAIN SYNAPTOSOMES AND CAT SUPERIOR CERVICAL GANGLION

B. COLLIER; S. LOVAT; D. ILSON; L. A. BARKER; T. W. MITTAG

doi:10.1111/j.1471-4159.1977.tb07752.x

THE UPTAKE, METABOLISM AND RELEASE OF HOMOCHOLINE: STUDIES WITH RAT BRAIN SYNAPTOSOMES AND CAT SUPERIOR CERVICAL GANGLION

B. COLLIER
, S. LOVAT
, D. ILSON
, L. A. BARKER
, T. W. MITTAG

Icahn School of Medicine at Mount Sinai

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

The accumulation of [³H]homocholine (3‐trimethylamino‐propan‐1‐01) by isolated synaptosomes prepared from rat brain was resolved kinetically into a high (K_T= 3.0 μM) and a low (K_T= 14.5 μM) affinity system. Although homocholine was not acetylated by solubilized choline acetyltransferase, 64% of the homocholine accumulated by intact synaptosomes via the high affinity uptake process was acetylated. Homocholine was also acetylated in the superior cervical ganglion of the cat, and the amount of acetylhomocholine formed was increased (12‐fold) by preganglionic nerve stimulation. In ganglia, acetylhomocholine was available for release by preganglionic nerve impulses, and its release was Ca²⁺‐dependent, It is concluded that homocholine can form a cholinergic false transmitter, and that the substrate specificity of choline acetyltransferase in vitro might be different from that in situ.

Original language	English
Pages (from-to)	331-339
Number of pages	9
Journal	Journal of Neurochemistry
Volume	28
Issue number	2
DOIs	https://doi.org/10.1111/j.1471-4159.1977.tb07752.x
State	Published - Feb 1977

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title = "THE UPTAKE, METABOLISM AND RELEASE OF HOMOCHOLINE: STUDIES WITH RAT BRAIN SYNAPTOSOMES AND CAT SUPERIOR CERVICAL GANGLION",

abstract = "The accumulation of [3H]homocholine (3‐trimethylamino‐propan‐1‐01) by isolated synaptosomes prepared from rat brain was resolved kinetically into a high (KT= 3.0 μM) and a low (KT= 14.5 μM) affinity system. Although homocholine was not acetylated by solubilized choline acetyltransferase, 64\% of the homocholine accumulated by intact synaptosomes via the high affinity uptake process was acetylated. Homocholine was also acetylated in the superior cervical ganglion of the cat, and the amount of acetylhomocholine formed was increased (12‐fold) by preganglionic nerve stimulation. In ganglia, acetylhomocholine was available for release by preganglionic nerve impulses, and its release was Ca2+‐dependent, It is concluded that homocholine can form a cholinergic false transmitter, and that the substrate specificity of choline acetyltransferase in vitro might be different from that in situ.",

author = "B. COLLIER and S. LOVAT and D. ILSON and BARKER, \{L. A.\} and MITTAG, \{T. W.\}",

year = "1977",

month = feb,

doi = "10.1111/j.1471-4159.1977.tb07752.x",

language = "English",

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T2 - STUDIES WITH RAT BRAIN SYNAPTOSOMES AND CAT SUPERIOR CERVICAL GANGLION

AU - COLLIER, B.

AU - LOVAT, S.

AU - ILSON, D.

AU - BARKER, L. A.

AU - MITTAG, T. W.

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N2 - The accumulation of [3H]homocholine (3‐trimethylamino‐propan‐1‐01) by isolated synaptosomes prepared from rat brain was resolved kinetically into a high (KT= 3.0 μM) and a low (KT= 14.5 μM) affinity system. Although homocholine was not acetylated by solubilized choline acetyltransferase, 64% of the homocholine accumulated by intact synaptosomes via the high affinity uptake process was acetylated. Homocholine was also acetylated in the superior cervical ganglion of the cat, and the amount of acetylhomocholine formed was increased (12‐fold) by preganglionic nerve stimulation. In ganglia, acetylhomocholine was available for release by preganglionic nerve impulses, and its release was Ca2+‐dependent, It is concluded that homocholine can form a cholinergic false transmitter, and that the substrate specificity of choline acetyltransferase in vitro might be different from that in situ.

AB - The accumulation of [3H]homocholine (3‐trimethylamino‐propan‐1‐01) by isolated synaptosomes prepared from rat brain was resolved kinetically into a high (KT= 3.0 μM) and a low (KT= 14.5 μM) affinity system. Although homocholine was not acetylated by solubilized choline acetyltransferase, 64% of the homocholine accumulated by intact synaptosomes via the high affinity uptake process was acetylated. Homocholine was also acetylated in the superior cervical ganglion of the cat, and the amount of acetylhomocholine formed was increased (12‐fold) by preganglionic nerve stimulation. In ganglia, acetylhomocholine was available for release by preganglionic nerve impulses, and its release was Ca2+‐dependent, It is concluded that homocholine can form a cholinergic false transmitter, and that the substrate specificity of choline acetyltransferase in vitro might be different from that in situ.

UR - https://www.scopus.com/pages/publications/0017641331

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DO - 10.1111/j.1471-4159.1977.tb07752.x

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JF - Journal of Neurochemistry

IS - 2

ER -

THE UPTAKE, METABOLISM AND RELEASE OF HOMOCHOLINE: STUDIES WITH RAT BRAIN SYNAPTOSOMES AND CAT SUPERIOR CERVICAL GANGLION

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